Anti-Oct-2 antibody (ab244486)

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Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody (ab244486)

    Key features and details

    • Rabbit polyclonal to Oct-2
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Oct-2 antibody
      See all Oct-2 primary antibodies

    • Description

      Rabbit polyclonal to Oct-2

    • Host species

      Rabbit

    • Tested applications

      Suitable for: ICC/IFmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Pig

    • Immunogen

      Recombinant fragment corresponding to Human Oct-2 aa 123-193.
      Sequence:

      PAQFLLPQAQQSQPGLLPTPNLFQLPQQTQGALLTSQPRAGLPTQAVTRP TLPDPHLSHPQPPKCLEPPSH


      Database link: P09086
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • ICC/IF: BJ cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.20
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 40% Glycerol (glycerin, glycerine)
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab244486 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF Use a concentration of 0.25 - 2 µg/ml.

    Fixation/Permeabilization: PFA/Triton X-100.

    Target

    • Function

      Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.

    • Tissue specificity

      Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.

    • Sequence similarities

      Belongs to the POU transcription factor family. Class-2 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.

    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • class 2 antibody
      • Lymphoid restricted immunoglobulin octamer binding protein antibody
      • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
      • NF A2 antibody
      • Oct 2 antibody
      • Oct-2 antibody
      • Octamer binding transcription factor 2 antibody
      • Octamer-binding protein 2 antibody
      • Octamer-binding transcription factor 2 antibody
      • OTF-2 antibody
      • OTF2 antibody
      • PO2F2_HUMAN antibody
      • POU domain antibody
      • POU domain class 2 transcription factor 2 antibody
      • POU2F2 antibody
      • transcription factor 2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody (ab244486)
      Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody (ab244486)

      PFA-fixed, Triton X-100 permeabilized BJ cells stained for Oct-2 (green) using ab244486 at 4 μg/ml in ICC/IF.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab244486? Please let us know so that we can cite the reference in this datasheet.

    ab244486 has not yet been referenced specifically in any publications.

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