Anti-Oct-2 antibody (ab244486)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Oct-2 antibody
See all Oct-2 primary antibodies -
Description
Rabbit polyclonal to Oct-2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Pig
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Immunogen
Recombinant fragment corresponding to Human Oct-2 aa 123-193.
Sequence:PAQFLLPQAQQSQPGLLPTPNLFQLPQQTQGALLTSQPRAGLPTQAVTRP TLPDPHLSHPQPPKCLEPPSH
Database link: P09086 -
Positive control
- ICC/IF: BJ cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab244486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. -
Tissue specificity
Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147. -
Sequence similarities
Belongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5452 Human
- Entrez Gene: 18987 Mouse
- Entrez Gene: 397105 Pig
- Omim: 164176 Human
- SwissProt: P09086 Human
- SwissProt: Q00196 Mouse
- SwissProt: Q29013 Pig
- Unigene: 147381 Human
see all -
Alternative names
- class 2 antibody
- Lymphoid restricted immunoglobulin octamer binding protein antibody
- Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Oct-2 antibody (ab244486)PFA-fixed, Triton X-100 permeabilized BJ cells stained for Oct-2 (green) using ab244486 at 4 μg/ml in ICC/IF.
Datasheets and documents
References
ab244486 has not yet been referenced specifically in any publications.
