Key features and details
- Rabbit polyclonal to Oct-2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Oct-2 antibody
See all Oct-2 primary antibodies
DescriptionRabbit polyclonal to Oct-2
SpecificityThis antibody recognises Oct-2. It may also detect the isoform 2, according to sequence analysis.
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide corresponding to Human Oct-2 (N terminal).
Database link: P09086
- WB: Raji cell lysate. IHC-P: Human lymph node tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Purification notesAffinity Purified
Our Abpromise guarantee covers the use of ab32156 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||Use at an assay dependent concentration.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTranscription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
Tissue specificityIsoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
Sequence similaritiesBelongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- class 2 antibody
- Lymphoid restricted immunoglobulin octamer binding protein antibody
- Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
Ab32156, at a 1:250 dilution, staining Oct-2 in paraffin embedded human lymph node tissue by Immunohistochemistry.
Anti-Oct-2 antibody (ab32156) at 1/1000 dilution + Raji cell lysate
Predicted band size: 51 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
ab32156 has been referenced in 1 publication.
- Rao S et al. AIF-regulated oxidative phosphorylation supports lung cancer development. Cell Res 29:579-591 (2019). PubMed: 31133695