Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Embryonic mouse midbrain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer
Permeabilization
Yes - PBS + 0.3% Triton X-100
Specification
Embryonic mouse midbrain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 1% BSA in PBS + 0.1% Triton X-100
Dilution
1/400
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: anti-rabbit Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Clear, strong and specific nuclear staining in subsets of neurons in the embryonic midbrain.
DAKO antigen retrieval buffer was used here, but presumably normal citrate buffer would work equally well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted May 06 2021