Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryonic mouse spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 1x DAKO antigen retrieval buffer
Permeabilization
Yes - 0.3% Triton X-100 in PBS for 5 minutes
Specification
embryonic mouse spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 1% BSA in PBS + 0.1% Triton X-100
Dilution
1/400
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: anti-rabbit Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Laura Lahti
Verified customer
Submitted Dec 04 2021