Overview

  • Product name

    Anti-Oct-2 antibody [EPR12482]
    See all Oct-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12482] to Oct-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Oct-2. The exact sequence is proprietary.
    Database link: P09086

  • Positive control

    • Ramos, Raji and Daudi cell lysates; Raji cells; Daudi cells.
  • General notes

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12482
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
    • Tissue specificity

      Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
    • Sequence similarities

      Belongs to the POU transcription factor family. Class-2 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • class 2 antibody
      • Lymphoid restricted immunoglobulin octamer binding protein antibody
      • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
      • NF A2 antibody
      • Oct 2 antibody
      • Oct-2 antibody
      • Octamer binding transcription factor 2 antibody
      • Octamer-binding protein 2 antibody
      • Octamer-binding transcription factor 2 antibody
      • OTF-2 antibody
      • OTF2 antibody
      • PO2F2_HUMAN antibody
      • POU domain antibody
      • POU domain class 2 transcription factor 2 antibody
      • POU2F2 antibody
      • transcription factor 2 antibody
      see all

    Images

    • All lanes : Anti-Oct-2 antibody [EPR12482] (ab179808) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 51 kDa

    • Immunofluorescent analysis of Raji cells labeling Oct-2 with ab179808 at 1:100.

    • Immunoprecipitation of Oct-2 from Raji cell lysate using ab179808 at 1:10 for IP (lane 1) or 1X PBS (negative control) (lane 2), and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Flow Cytometrical analysis of permeabilized Daudi cells labeling Oct-2 with ab179808 at 1:100 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Gao H  et al. Omeprazole protects against cisplatin-induced nephrotoxicity by alleviating oxidative stress, inflammation, and transporter-mediated cisplatin accumulation in rats and HK-2?cells. Chem Biol Interact 297:130-140 (2019). Read more (PubMed: 30452898) »
    • Song H  et al. BRD4 promotes the stemness of gastric cancer cells via attenuating miR-216a-3p-mediated inhibition of Wnt/ß-catenin signaling. Eur J Pharmacol 852:189-197 (2019). Read more (PubMed: 30876979) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Embryonic spinal cord)
    Permeabilization
    Yes - 0.1%Triton X-100
    Specification
    Embryonic spinal cord
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde

    Dr. Frederic Clotman

    Verified customer

    Submitted Jun 10 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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