Immunohistochemistry (Frozen sections) abreview for Anti-Oct-2 antibody [EPR12482]

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic spinal cord)
Permeabilization
Yes - 0.1%Triton X-100
Specification
Embryonic spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/10% horse serum/0.1% TritonX-100
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-rabbit AlexaFluor 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
Several dilutions (1/100 to 1/10.000) and antigen retrieval procedure have been tested, without detection of any specific signal
Abcam response
Thank you for participating in our AbTrial testing program. We appreciate you taking the time to submit this review. Based on your data, it appears that this antibody may not be suitable with this combination of application and species.

Dr. Frederic Clotman

Verified customer

Submitted Jun 10 2016

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