Overview

  • Product name

    Anti-Oct-2 antibody [EPR12482] (HRP)
    See all Oct-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12482] to Oct-2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Oct-2. The exact sequence is proprietary.
    Database link: P09086

  • Positive control

    • WB: Ramos, Raji, Daudi cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: 1% BSA, 30% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12482
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
  • Tissue specificity

    Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
  • Sequence similarities

    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • class 2 antibody
    • Lymphoid restricted immunoglobulin octamer binding protein antibody
    • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
    • NF A2 antibody
    • Oct 2 antibody
    • Oct-2 antibody
    • Octamer binding transcription factor 2 antibody
    • Octamer-binding protein 2 antibody
    • Octamer-binding transcription factor 2 antibody
    • OTF-2 antibody
    • OTF2 antibody
    • PO2F2_HUMAN antibody
    • POU domain antibody
    • POU domain class 2 transcription factor 2 antibody
    • POU2F2 antibody
    • transcription factor 2 antibody
    see all

Images

  • All lanes : Anti-Oct-2 antibody [EPR12482] (HRP) (ab205793) at 1/1000 dilution

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 51 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205793 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab205793 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab205793.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up