Recombinant HRP Anti-Oct-2 antibody [EPR12482] (ab205793)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR12482] to Oct-2
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
-
Product name
HRP Anti-Oct-2 antibody [EPR12482]
See all Oct-2 primary antibodies -
Description
HRP Rabbit monoclonal [EPR12482] to Oct-2 -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human Oct-2. The exact sequence is proprietary.
Database link: P09086 -
Positive control
- WB: Ramos, Raji, Daudi cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12482 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).
|
Notes |
---|
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa). |
Target
-
Function
Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter. -
Tissue specificity
Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147. -
Sequence similarities
Belongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5452 Human
- Entrez Gene: 18987 Mouse
- Entrez Gene: 117058 Rat
- Omim: 164176 Human
- SwissProt: P09086 Human
- SwissProt: Q00196 Mouse
- Unigene: 147381 Human
- Unigene: 76683 Mouse
-
Alternative names
- class 2 antibody
- Lymphoid restricted immunoglobulin octamer binding protein antibody
- Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
see all
Images
-
All lanes : HRP Anti-Oct-2 antibody [EPR12482] (ab205793) at 1/1000 dilution
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : Daudi (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205793 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab205793 has not yet been referenced specifically in any publications.