Product nameAnti-Oct-2 antibody [EPR542]
See all Oct-2 primary antibodies
DescriptionRabbit monoclonal [EPR542] to Oct-2
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human Oct-2 aa 150-250. The exact sequence is proprietary.
Database link: P09086
- WB: Daudi cell lysate. IHC-P: Human tonsil tisue. Flow Cyt: Raji cells.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab92458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: 1/250 - 1/500.
IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
WB: 1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
Tissue specificityIsoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
Sequence similaritiesBelongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- class 2 antibody
- Lymphoid restricted immunoglobulin octamer binding protein antibody
- Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
Anti-Oct-2 antibody [EPR542] (ab92458) at 1/1000 dilution + Daudi (Human Burkitt's lymphoma cell line) lysate at 10 µg
HRP conjugated goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
ab92458 at a 1:250 dilution staining Oct-2 in human tonsil (paraffin embedded section) by immunohistochemistry.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Overlay histogram showing Raji (Human Burkitt's lymphoma cell line) cells stained with ab92458 (red line).
The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92458, 1:50) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1:500 dilution for 30 min at 22ºC.
Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
ab92458 has not yet been referenced specifically in any publications.