Overview

  • Product name

    Anti-Oct-2 antibody [EPR542] - BSA and Azide free
    See all Oct-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR542] to Oct-2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Oct-2 aa 150-250. The exact sequence is proprietary.
    Database link: P09086

  • General notes

    ab239913 is the carrier-free version of ab92458 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239913 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 51 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
    • Tissue specificity

      Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
    • Sequence similarities

      Belongs to the POU transcription factor family. Class-2 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • class 2 antibody
      • Lymphoid restricted immunoglobulin octamer binding protein antibody
      • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
      • NF A2 antibody
      • Oct 2 antibody
      • Oct-2 antibody
      • Octamer binding transcription factor 2 antibody
      • Octamer-binding protein 2 antibody
      • Octamer-binding transcription factor 2 antibody
      • OTF-2 antibody
      • OTF2 antibody
      • PO2F2_HUMAN antibody
      • POU domain antibody
      • POU domain class 2 transcription factor 2 antibody
      • POU2F2 antibody
      • transcription factor 2 antibody
      see all

    Images

    • ab92458, at 1:250 staining Oct-2 in Human tonsil paraffin embeeded section by immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92458).

    • Overlay histogram showing Raji cells stained with ab92458 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92458, 1:50) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1:500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92458).

    References

    ab239913 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239913.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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