Overview

  • Product name

    Anti-Oct-2 antibody [OCT2/2137]
    See all Oct-2 primary antibodies
  • Description

    Mouse monoclonal [OCT2/2137] to Oct-2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Oct-2 aa 112-297. The exact sequence is proprietary.
    Database link: P09086

  • Positive control

    • WB: Ramos and Daudi cell lysate; Recombinant Oct-2 protein. IHC-P: Human lymph node and tonsil tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab236558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary antibody for 30 minutes at RT.

Protein Array Use at an assay dependent concentration.

Target

  • Function

    Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
  • Tissue specificity

    Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
  • Sequence similarities

    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • class 2 antibody
    • Lymphoid restricted immunoglobulin octamer binding protein antibody
    • Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
    • NF A2 antibody
    • Oct 2 antibody
    • Oct-2 antibody
    • Octamer binding transcription factor 2 antibody
    • Octamer-binding protein 2 antibody
    • Octamer-binding transcription factor 2 antibody
    • OTF-2 antibody
    • OTF2 antibody
    • PO2F2_HUMAN antibody
    • POU domain antibody
    • POU domain class 2 transcription factor 2 antibody
    • POU2F2 antibody
    • transcription factor 2 antibody
    see all

Images

  • ab236558 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Anti-Oct-2 antibody [OCT2/2137] (ab236558) at 2 µg/ml + Ramos (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 51 kDa

  • Formalin-fixed, paraffin-embedded human lymph node tissue stained for Oct-2 using ab236558 at 2 µg/ml in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Oct-2 using ab236558 at 2 µg/ml in immunohistochemical analysis.

  • All lanes : Anti-Oct-2 antibody [OCT2/2137] (ab236558) at 2 µg/ml

    Lane 1 : Recombinant Oct-2 protein
    Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 51 kDa

References

ab236558 has not yet been referenced specifically in any publications.

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