Key features and details
- Mouse monoclonal [OCT2/2137] to Oct-2
- Suitable for: WB, IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Oct-2 antibody [OCT2/2137]
See all Oct-2 primary antibodies
DescriptionMouse monoclonal [OCT2/2137] to Oct-2
Tested applicationsSuitable for: WB, IHC-P, Protein Arraymore details
Species reactivityReacts with: Human
Recombinant fragment within Human Oct-2 aa 112-297. The exact sequence is proprietary.
Database link: P09086
- WB: Ramos and Daudi cell lysate; Recombinant Oct-2 protein. IHC-P: Human lymph node and tonsil tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from bioreactor concentrate by Protein A/G.
Light chain typekappa
Our Abpromise guarantee covers the use of ab236558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at RT.
|Protein Array||Use at an assay dependent concentration.|
FunctionTranscription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
Tissue specificityIsoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
Sequence similaritiesBelongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- class 2 antibody
- Lymphoid restricted immunoglobulin octamer binding protein antibody
- Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2 antibody
ab236558 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Anti-Oct-2 antibody [OCT2/2137] (ab236558) at 2 µg/ml + Ramos (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 51 kDa
Formalin-fixed, paraffin-embedded human lymph node tissue stained for Oct-2 using ab236558 at 2 µg/ml in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Oct-2 using ab236558 at 2 µg/ml in immunohistochemical analysis.
All lanes : Anti-Oct-2 antibody [OCT2/2137] (ab236558) at 2 µg/ml
Lane 1 : Recombinant Oct-2 protein
Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 51 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236558 has not yet been referenced specifically in any publications.