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    oct4-antibody-ab134218.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families OCT
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Anti-Oct4 antibody (ab134218)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab134218)
  • Western blot - Anti-Oct4 antibody (ab134218)

Key features and details

  • Chicken polyclonal to Oct4
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgY

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Primary
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Anti-ATG9A antibody [EPR2450(2)] (ab108338)
Secondary
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Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Protein
Recombinant human Oct4 protein (ab169842)

View more associated products

Overview

  • Product name

    Anti-Oct4 antibody
    See all Oct4 primary antibodies
  • Description

    Chicken polyclonal to Oct4
  • Host species

    Chicken
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Oct4 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: E14Tg2a; F9. IF/ICC: mES cell line.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: 3% BSA

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • Purity

    Protein L purified
  • Purification notes

    Affinity Purified
  • Clonality

    Polyclonal
  • Isotype

    IgY
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • OCT
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules

Associated products

  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)
  • Recombinant Protein

    • Recombinant human Oct4 protein (ab169842)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
All applications
Rat
Rabbit
Pig
Chimpanzee
Chinese hamster
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
ICC/IF (1)
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Tissue specificity

    Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • Sequence similarities

    Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Developmental stage

    Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • Domain

    The POU-specific domain mediates interaction with PKM2.
  • Post-translational
    modifications

    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • Cellular localization

    Nucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Target information above from: UniProt accession Q01860 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 744263 Chimpanzee
    • Entrez Gene: 5460 Human
    • Entrez Gene: 18999 Mouse
    • Entrez Gene: 100127461 Pig
    • Entrez Gene: 100101567 Rabbit
    • Entrez Gene: 294562 Rat
    • Omim: 164177 Human
    • SwissProt: Q7YR49 Chimpanzee
    • SwissProt: Q01860 Human
    • SwissProt: P20263 Mouse
    • SwissProt: Q9TSV5 Pig
    • Unigene: 249184 Human
    • Unigene: 632482 Human
    • Unigene: 646545 Human
    • Unigene: 17031 Mouse
    • Unigene: 161748 Rat
    see all
  • Alternative names

    • Octamer binding transcription factor 4 antibody
    • MGC22487 antibody
    • Oct 3 antibody
    • Oct 4 antibody
    • Oct-3 antibody
    • Oct-4 antibody
    • OCT3 antibody
    • Oct4 antibody
    • Octamer binding protein 3 antibody
    • Octamer binding protein 4 antibody
    • Octamer binding transcription factor 3 antibody
    • Octamer-binding protein 3 antibody
    • Octamer-binding protein 4 antibody
    • Octamer-binding transcription factor 3 antibody
    • OTF 3 antibody
    • OTF 4 antibody
    • OTF-3 antibody
    • OTF3 antibody
    • OTF4 antibody
    • PO5F1_HUMAN antibody
    • POU class 5 homeobox 1 antibody
    • POU domain class 5 transcription factor 1 antibody
    • POU domain transcription factor OCT4 antibody
    • POU domain, class 5, transcription factor 1 antibody
    • POU-type homeodomain-containing DNA-binding protein antibody
    • POU5F1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab134218)
    Immunocytochemistry/ Immunofluorescence - Anti-Oct4 antibody (ab134218)

    ICC/IF image of ab134218 stained mES cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab134218, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96947, DyLight® 488 goat anti-chicken IgY (H+L) used at a 1:250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Western blot - Anti-Oct4 antibody (ab134218)
    Western blot - Anti-Oct4 antibody (ab134218)
    All lanes : Anti-Oct4 antibody (ab134218) at 1 µg/ml

    Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab134218? Please let us know so that we can cite the reference in this datasheet.

ab134218 has been referenced in 3 publications.

  • He Z  et al. Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 to downregulate ENAH and thus retard esophageal squamous cell carcinoma progression. J Exp Clin Cancer Res 39:140 (2020). PubMed: 32698859
  • Dong H  et al. GLI1 activation by non-classical pathway integrin avß3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death Dis 10:574 (2019). PubMed: 31366904
  • Huang H  et al. A Novel Approach for Meniscal Regeneration Using Kartogenin-Treated Autologous Tendon Graft. Am J Sports Med 45:3289-3297 (2017). PubMed: 28859517

Customer reviews and Q&As

Show All Reviews Q&A
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Immunocytochemistry/ Immunofluorescence abreview for Anti-Oct4 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Induced Pluripotent Stem Cells)
Permeabilization
Yes - 0.25% Triton-X 100 for 5 min.
Specification
Human Induced Pluripotent Stem Cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

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Verified customer

Submitted Aug 22 2016

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