Overview

  • Product name

  • Description

    Rabbit polyclonal to Oct4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Cow, Cat, Pig, Monkey
  • Immunogen

    Recombinant fragment corresponding to Human Oct4 aa 1-182.

  • Positive control

    • Recombinant human Oct4 protein (ab134876) can be used as a positive control in WB. HeLa, Human embryonic stem cells (ESC) and Mouse ESC whole cell lysates; HeLa nuclear cell lysate; Human embryonic stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
Flow Cyt 1/50 - 1/200.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/1000.

Target

  • Function

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Tissue specificity

    Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
  • Sequence similarities

    Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Developmental stage

    Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
  • Domain

    The POU-specific domain mediates interaction with PKM2.
  • Post-translational
    modifications

    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
  • Cellular localization

    Nucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt
  • Database links

  • Alternative names

    • Octamer binding transcription factor 4 antibody
    • MGC22487 antibody
    • Oct 3 antibody
    • Oct 4 antibody
    • Oct-3 antibody
    • Oct-4 antibody
    • OCT3 antibody
    • Oct4 antibody
    • Octamer binding protein 3 antibody
    • Octamer binding protein 4 antibody
    • Octamer binding transcription factor 3 antibody
    • Octamer-binding protein 3 antibody
    • Octamer-binding protein 4 antibody
    • Octamer-binding transcription factor 3 antibody
    • OTF 3 antibody
    • OTF 4 antibody
    • OTF-3 antibody
    • OTF3 antibody
    • OTF4 antibody
    • PO5F1_HUMAN antibody
    • POU class 5 homeobox 1 antibody
    • POU domain class 5 transcription factor 1 antibody
    • POU domain transcription factor OCT4 antibody
    • POU domain, class 5, transcription factor 1 antibody
    • POU-type homeodomain-containing DNA-binding protein antibody
    • POU5F1 antibody
    see all

Images

  • Anti-Oct4 antibody (ab137427) at 1/5000 dilution + Mouse Embryonic Stem Cell whole cell lysate
    at 20 µg

    Predicted band size: 38 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:400 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:200 dilution.

  • Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:100 dilution. Lower image shows cells co-stained with DAPI.

  • Anti-Oct4 antibody (ab137427) at 1/1000 dilution + HeLa nuclear cell lysate at 20 µg

    Predicted band size: 38 kDa



    10% SDS PAGE
  • Anti-Oct4 antibody (ab137427) at 1/1000 dilution + Human Embryonic Stem Cell whole cell lysate at 20 µg

    Predicted band size: 38 kDa



    10% SDS PAGE
  • Flow cytometric analysis of Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:50 dilution (green) or rabbit IgG (black).

References

This product has been referenced in:

  • Lima DBC  et al. Influence of warming and reanimation conditions on seminiferous tubule morphology, mitochondrial activity, and cell composition of vitrified testicular tissues in the domestic cat model. PLoS One 13:e0207317 (2018). Read more (PubMed: 30408126) »
  • Jia D  et al. An autocrine inflammatory forward-feedback loop after chemotherapy withdrawal facilitates the repopulation of drug-resistant breast cancer cells. Cell Death Dis 8:e2932 (2017). Read more (PubMed: 28703802) »
See all 6 Publications for this product

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