Key features and details
- Rabbit polyclonal to Oct4
- Suitable for: IP
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Oct4 antibody
See all Oct4 primary antibodies
DescriptionRabbit polyclonal to Oct4
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Synthetic peptide within Human Oct4 aa 250-300. The exact sequence is proprietary. NP_038661.1
Database link: Q01860-1
- IP: F9 whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab265606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Use at 2-10 µg/mg of lysate.
FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
DomainThe POU-specific domain mediates interaction with PKM2.
modificationsSumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.
- Information by UniProt
- Octamer binding transcription factor 4 antibody
- MGC22487 antibody
- Oct 3 antibody
Oct4 was immunoprecipitated from 1mg of F9 whole cell lysate with ab265606 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using a different antibody.
Lane 1: ab265606 IP in F9 whole cell lysate.
Lane 2: Control IgG.
Exposure time: 3 mins.
Cells prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265606 has not yet been referenced specifically in any publications.