Product nameAnti-Oct4 antibody
See all Oct4 primary antibodies
DescriptionGoat polyclonal to Oct4
SpecificityAccording to the UniProt database Oct4 is mainly localized in the nucleus but can also occur in the cytoplasm: UniProtKB - Q01860
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Oct4 aa 300 to the C-terminus (C terminal).
(Peptide available as
- Human or Mouse Embryonic Stem Cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
Concentration information loading...
PurityProtein G purified
Purification notesAffinity Purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab27985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 39 kDa).|
FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
DomainThe POU-specific domain mediates interaction with PKM2.
modificationsSumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.
- Information by UniProt
- Octamer binding transcription factor 4 antibody
- MGC22487 antibody
- Oct 3 antibody
ab27985 recognizes Oct4 in human embryonic stem cells. The upper panel shows DAPI staining while the lower panel shows ab27985 staining.
All lanes : Anti-Oct4 antibody (ab27985) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat IgG secondary antibody (ab28445) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
The antibody recognised a band corresponding to Oct4 in mouse and human embryonic stem cell lysates.
ab27985 staining Oct4 in human embryonic carcinoma cells by Flow Cytometery. Cells were fixed in paraformaldehyde and permeabilized in saponin buffer. The primary antibody was diluted 1:500 (PBS + 10% BSA) and incubated with sample for 1 hour at 4°C. A FITC conjugated Donkey polyclonal to goat IgG, diluted 1:200 was used as secondary. The red histogram is secondary only control whilst green and blue repersents Oct4 expression in untreated and partially differentiated with 10nM Trans-Retinoic acid for 14 days.
ab27985 recognizes Oct4 in mouse embryonic stem cells. DAPI staining is shown in the top panel while ab27985 staining is shown in the lower panel.
This product has been referenced in:
- Peng Y et al. Human embryonic stem cells extracellular vesicles and their effects on immortalized human retinal Müller cells. PLoS One 13:e0194004 (2018). Read more (PubMed: 29538408) »
- Orsztynowicz M et al. Changes in chromosome territory position within the nucleus reflect alternations in gene expression related to embryonic lineage specification. PLoS One 12:e0182398 (2017). Read more (PubMed: 28767705) »