Recombinant
RabMAb

Anti-Oct4 antibody [EPR2054] (ab109183)

Overview

  • Product name
    Anti-Oct4 antibody [EPR2054]
    See all Oct4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2054] to Oct4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Oct4 aa 250-350. The exact sequence is proprietary.

  • Positive control
    • WB: NCCIT and HeLa cell lysates IHC-P:Human Seminoma, Human ovarian dysgerminoma tissue
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for ICC.
  • Target

    • Function
      Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
    • Tissue specificity
      Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
    • Sequence similarities
      Belongs to the POU transcription factor family. Class-5 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Developmental stage
      Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
    • Domain
      The POU-specific domain mediates interaction with PKM2.
    • Post-translational
      modifications
      Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
      Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
    • Cellular localization
      Nucleus. Expressed in a diffuse and slightly punctuate pattern.
    • Information by UniProt
    • Database links
    • Alternative names
      • Octamer binding transcription factor 4 antibody
      • MGC22487 antibody
      • Oct 3 antibody
      • Oct 4 antibody
      • Oct-3 antibody
      • Oct-4 antibody
      • OCT3 antibody
      • Oct4 antibody
      • Octamer binding protein 3 antibody
      • Octamer binding protein 4 antibody
      • Octamer binding transcription factor 3 antibody
      • Octamer-binding protein 3 antibody
      • Octamer-binding protein 4 antibody
      • Octamer-binding transcription factor 3 antibody
      • OTF 3 antibody
      • OTF 4 antibody
      • OTF-3 antibody
      • OTF3 antibody
      • OTF4 antibody
      • PO5F1_HUMAN antibody
      • POU class 5 homeobox 1 antibody
      • POU domain class 5 transcription factor 1 antibody
      • POU domain transcription factor OCT4 antibody
      • POU domain, class 5, transcription factor 1 antibody
      • POU-type homeodomain-containing DNA-binding protein antibody
      • POU5F1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human seminoma tissue sections labeling Oct4 - with Purified ab109183 at 1:600 dilution (1 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
    • Anti-Oct4 antibody [EPR2054] (ab109183) at 0.06 µg/ml (purified) + NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 39 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-Oct4 antibody [EPR2054] (ab109183) at 1/1000 dilution (Unpurified)

      Lane 1 : Seminoma lysate
      Lane 2 : NCCIT cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    • Unpurified ab109183, at 1:500 dilution, staining Oct4 in paraffin-embedded Human ovarian dysgerminoma by Immunohistochemistry.

    • Unpurified ab109183 at 1/50 immunoprecipitating Oct4 in NCCIT (human pluripotent embryonal carcinoma) whole cell lysate observed at 39 KDa (lanes 1 and 2).

      Lane 1 (input): NCCIT whole cell lysate, 10μg

      Lane 2 (+): ab109183 + NCCIT whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109183 in NCCIT whole cell lysate

      For western blotting, ab109183 at 1/1000 dilution and ab131366 VeriBlot for IP (HRP) was used as the secondary antibody at 1/10000.

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

       

       

    • Unpurified ab109183 showing negative staining in Colonic adenocarcinoma tissue.

    • Unpurified ab109183 showing negative staining in Skeletal muscle tissue.

    • Unpurified ab109183 showing negative staining in Normal liver tissue.

    • Unpurified ab109183 showing positive staining in Seminoma tissue.

    • Unpurified ab109183 showing negative staining in Normal brain tissue.

    • Unpurified ab109183 showing positive staining in Fetal brain tissue.

    • Unpurified ab109183 showing negative staining in Hepatocellular carcinoma tissue.

    • Unpurified ab109183 showing positive staining in Embryonal carcinoma tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Zhou Y  et al. Endogenous authentic OCT4A proteins directly regulate FOS/AP-1 transcription in somatic cancer cells. Cell Death Dis 9:585 (2018). Read more (PubMed: 29789579) »
    • Ram RS  et al. Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin-Angiotensin System. Front Surg 4:30 (2017). IHC ; Human . Read more (PubMed: 28634582) »

    See all 10 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Purified protein (brain cancer)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    brain cancer
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 24 2015

    Thank you for your enquiry and your interest.

    I can confirm that two isoforms of Oct 4 have been identified, and the exact amino acid sequences (aa) can be found at this website: http://www.uniprot.org/uniprot/Q01860

    - Isoform A: Q0...

    Read More

    Muchas gracias por tu respuesta.

    Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Est...

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    Thank you for contacting Abcam. Regarding ab74538, when stored properly at -20, the antibody is expected to be stable for up to 5 years from receipt. I hope this information is helpful. Please do not hesitate to contact us if you have any additional qu...

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    Thank you for contacting Abcam regarding ab109183. I am sorry to hear that you have been experiencing difficulties with this antibody. Can you please provide some additional details as to the nature of the problems you are experiencing and your protoco...

    Read More

    Thank you for contacting Abcam about this. I have changed the datasheet to remedy the error that you have noticed. Ab109183 Anti-Oct4 antibody [EPR2054] is a rabbit IgG isotype. When using in Western Blot I would recommend using one of the followin...

    Read More

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