Recombinant
RabMAb

Recombinant Anti-Oct4 antibody [EPR2054] (ab109183)

Reviews (1)Q&A (5)References (19)

Overview

  • Product name

    Anti-Oct4 antibody [EPR2054]
    See all Oct4 primary antibodies

  • Description

    Rabbit monoclonal [EPR2054] to Oct4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Oct4 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • WB: NCCIT and HeLa whole cell lysate (ab150035). IHC-P:Human seminoma and ovarian dysgerminoma tissue.

  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for ICC.

    • Target

    • Function

      Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

    • Tissue specificity

      Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

    • Sequence similarities

      Belongs to the POU transcription factor family. Class-5 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.

    • Developmental stage

      Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.

    • Domain

      The POU-specific domain mediates interaction with PKM2.

    • Post-translational
      modifications

      Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
      Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.

    • Cellular localization

      Nucleus. Expressed in a diffuse and slightly punctuate pattern.
    • Information by UniProt

    • Database links

      • Alternative names

        • Octamer binding transcription factor 4 antibody
        • MGC22487 antibody
        • Oct 3 antibody
        • Oct 4 antibody
        • Oct-3 antibody
        • Oct-4 antibody
        • OCT3 antibody
        • Oct4 antibody
        • Octamer binding protein 3 antibody
        • Octamer binding protein 4 antibody
        • Octamer binding transcription factor 3 antibody
        • Octamer-binding protein 3 antibody
        • Octamer-binding protein 4 antibody
        • Octamer-binding transcription factor 3 antibody
        • OTF 3 antibody
        • OTF 4 antibody
        • OTF-3 antibody
        • OTF3 antibody
        • OTF4 antibody
        • PO5F1_HUMAN antibody
        • POU class 5 homeobox 1 antibody
        • POU domain class 5 transcription factor 1 antibody
        • POU domain transcription factor OCT4 antibody
        • POU domain, class 5, transcription factor 1 antibody
        • POU-type homeodomain-containing DNA-binding protein antibody
        • POU5F1 antibody
        see all

      Images

      • Western blot - Anti-Oct4 antibody [EPR2054] (ab109183)
        Western blot - Anti-Oct4 antibody [EPR2054] (ab109183)
        Anti-Oct4 antibody [EPR2054] (ab109183) at 0.06 µg/ml (purified) + NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 39 kDa



        Blocking and diluting buffer: 5% NFDM/TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human seminoma tissue sections labeling Oct4 - with Purified ab109183 at 1:600 dilution (1 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
      • Western blot - Anti-Oct4 antibody [EPR2054] (ab109183)
        Western blot - Anti-Oct4 antibody [EPR2054] (ab109183)
        All lanes : Anti-Oct4 antibody [EPR2054] (ab109183) at 1/1000 dilution (Unpurified)

        Lane 1 : Seminoma lysate
        Lane 2 : NCCIT cell lysate
        Lane 3 : HeLa cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 39 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183, at 1:500 dilution, staining Oct4 in paraffin-embedded Human ovarian dysgerminoma by Immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunoprecipitation - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183 at 1/50 immunoprecipitating Oct4 in NCCIT (human pluripotent embryonal carcinoma) whole cell lysate observed at 39 KDa (lanes 1 and 2).

        Lane 1 (input): NCCIT whole cell lysate, 10μg

        Lane 2 (+): ab109183 + NCCIT whole cell lysate

        Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109183 in NCCIT whole cell lysate

        For western blotting, ab109183 at 1/1000 dilution and VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

        Blocking buffer and concentration: 5% NFDM/TBST.
        Diluting buffer and concentration: 5% NFDM /TBST.

         

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183 showing negative staining in Colonic adenocarcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183 showing negative staining in Skeletal muscle tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183 showing negative staining in Normal liver tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183 showing positive staining in Seminoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183 showing negative staining in Normal brain tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183 showing positive staining in Fetal brain tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183 showing negative staining in Hepatocellular carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct4 antibody [EPR2054] (ab109183)

        Unpurified ab109183 showing positive staining in Embryonal carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Other - Anti-Oct4 antibody [EPR2054] (ab109183)
        Other - Anti-Oct4 antibody [EPR2054] (ab109183)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      • Kaowinn S  et al. CGK062, a small chemical molecule, inhibits cancer upregulated gene 2-induced oncogenesis through NEK2 and ß-catenin. Int J Oncol 54:1295-1305 (2019). Read more (PubMed: 30968157) »
      • Zhou Y  et al. Endogenous authentic OCT4A proteins directly regulate FOS/AP-1 transcription in somatic cancer cells. Cell Death Dis 9:585 (2018). Read more (PubMed: 29789579) »
      See all 19 Publications for this product

      Publishing research using ab109183? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-Oct4 antibody [EPR2054]

       Excellent
      Abreviews
      Application
      Western blot
      Sample
      Human Purified protein (brain cancer)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Specification
      brain cancer
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 24 2015

      Question

      Dear Sir / Madam,
      One of the biggest customer in stem cell lab would like to have an
      antibody that is specific against Oct 4, either variant A or variant B
      to distinguish between them. In literature, oct 4 antibodies are not
      able to bind to specific variants.
      What do you recommend?
      Many Thanks and Best Regards,

      Read More
      Answer

      Thank you for your enquiry and your interest.

      I can confirm that two isoforms of Oct 4 have been identified, and the exact amino acid sequences (aa) can be found at this website: http://www.uniprot.org/uniprot/Q01860

      - Isoform A: Q01860-1 (1-360 aa)

      - Isoform B: Q01860-2 (1-265 aa)

      ab109183 - this antibody was raised against a peptide corresponding to the region between 265-360 aa, soit is very likely to recognize Isoform A.

      ab18976 - the immunogen selected was around region 1-14 aa, thus it would recognize potentially both Isofm A and B.

      If you need any further assistance in the future, please do not hesitate to contact me.

      Read More

      Question

      Hola ,
      ¿Qué tal? estoy interesada en inmunofluorescencia
      de doble marcaje en tejidos humanos parafinados.
      En concreto me interesarían los anticuerpos primarios, a ser posible
      monoclonales, de distintas especies para poder combinarlos, contra las
      siguientes proteínas humanas:
      * OCT4: ab18976, ab109183, ab129030
      * tenascin C: ab6346, ab3970, ab108930, ab86182
      * CD68: ab955, ab845, ab125047
      * S100A6: ab11181, ab117714, ab75676
      * ganglioside GD2: ab68456
      * Sox10: ab107532, ab122977
      * MMP9: ab76003, ab119906
      * CD163: ab111250, ab100909
      * PNMT: ab119784
      He indicado los productos que me parecían interesantes de la web, pero
      acepto sugerencias.
      Y tambíen estaría interesada en los anticuerpos secundarios marcados
      con distintos fluorocromos para anticuerpos de distintas especies.
      Necesitaría la ficha técnica y el precio de cada uno de ellos.
      Muchas gracias.
      Saludos,

      Read More
      Answer

      Muchas gracias por tu respuesta.

      Es importante que todos los anticuerpos que escojas para llevar a cabo IHC-P en tejido humano estén validados (y por tanto garantizados por Abcam) para funcionar en esta especie y aplicación. Esta información aparece en la hoja técnica de los productos.

      También es importante echar un vistazo a las hojas de datos de cada uno de los productos para tener en cuenta recomendaciones especificas, tales como diluciones, métodos de recuperación antigénica, y demás que pueden aparecer en ellas. A las fichas técnicas se puede acceder desde la página web, escribiendo el código del anticuerpo (ab955, por ejemplo) en la casilla azul en la parte de arriba de nuestra página web (https://www.abcam.com/).

      Desconozco las combinaciones que queréis llevar a cabo mediante inmunofluorescencia doble con los anticuerpos mencionados. En general, a la hora de hacer inmunoensayos múltiples hay que tener mucha precaución para evitar reactividad cruzada. Es importante usar anticuerpos procedentes de distintas especies si se puede, o al menos con diferentes isotipos. Una buena forma de evitar reactividad cruzada es usando secundarios de la misma especie. Además esto permite usar suero de dicha especie para bloquear.

      Te copio los links a los protocolos que tenemos en nuestra web para llevar a cabo doble inmunofluorescencia por s pudieran resultar de ayuda:

      https://www.abcam.com/index.html?pageconfig=resource&rid=11459

      https://www.abcam.com/index.html?pageconfig=resource&rid=11458

      De todas maneras si quieres que comentemos distintas posibilidades y escenarios posibles, no dudes en contactarme otra vez.

      Respecto a los secundarios, tenemos muchos anticuerpos que podrían serviros. Te aconsejo que en función de los primarios y combinaciones que vayáis a llevar a cabo, optéis por uno u otro. Para llevar a cabo la búsqueda de los secundarios desde la pagina web (https://www.abcam.com/) pincha en la pestaña “Secondary Antibodies”. Se abre una nueva ventana de “Advance Search”, en la cual se pueden elegir las categorías requeridas. La única categoría obligatoria es el isotipo del anticuerpo, pues tiene que coincidir con el del primario. El resto de categorías son opcionales, pero son muy útiles para afinar la búsqueda y elegir el anticuerpo más apropiado según la especie del primario, la conjugación deseada, la clonalidad, la aplicación en la que se va a testar, etc…

      Los precios y la disponibilidad pueden consultarse igualmente en la parte superior de la datasheet de los productos. Si necesitas un presupuesto no dudes en hacérmelo saber para que te lo envíe. Además tenemos algún descuento para pedidos superiores a 5 productos, por lo que si vais a realizar un pedido de estas características te animaría a que nos contactaras primero.

      Espero que esta información sea de ayuda. Como te digo, para cualquier aclaración o más sugerencias, estaré encantada de echar una mano.

      Read More

      Question

      I am a dealer in China, one of Our customer want purchase a Oct4 antibody：ab74538，they wanna know the product date of this antibody. It will be very helpful if you can tell me these information. Yours Ge Hao At 2012-01-06 05:07:05,technical@abcam.com wrote: body { margin: 0px; margin-top: 10px; font-family: arial, verdana, tahoma; background-color: #F5F5EE; p{ padding-top: 4px; margin: 0px; line-height: 18px; body table{ font-size: 12px; line-height: 100%; a { text-decoration: underline; color: #004a91; a:visited { color: #0096db; a:hover { color: #0096db; text-decoration: underline; .mainText { margin-left: 0cm; font-weight: normal; margin-left: 10px; .tagLine { margin-left: 0cm; font-weight: normal; margin-left: 10px; color: #FFFFFF; .tagLine a { margin-left: 0cm; font-weight: normal; text-decoration: none; color: #FFFFFF; .tagLink { margin-left: 0cm; font-weight: normal; text-decoration: none; color: #FFFFFF; font-weight: bold; .salutation{ margin-left: 0cm; padding-bottom: 10px; font-weight: bold; margin-left: 10px; https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header Dear Xinjiang We have an answer to your inquiry: Thank you for contacting Abcam regarding ab109183. I am sorry to hear that you have been experiencing difficulties with this antibody. Can you please provide some additional details as to the nature of the problems you are experiencing and your protocol? I am happy to help you in any way that I can. I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions. Help us improve our service. https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3402261 Your original inquiry to Abcam: hello,Dear abcam company, hello! I have a production of antibodies when the problem is anti-Oct4antibody [EPR2054] (ab109183), hope to get answers, thank you very much! yours Good: smooth, all is well! Thanks and greetings! MR. Ge Hao Customer Sales Representative Sales and technical baoxin (Xinjiang) Co., Ltd. Sales Urumqi, Xinjiang Medical University, Room 620, 6th Floor （830054） Tel： +86 (0991)4311457 FAX： +86 (0991)4312752 Ishikawa City University School of Medicine 32 Shihezi sub-district 2-151 （832003） Mobile：15999299363 Tel： +86 (0993)6668003 FAX: +86 (0993)6668003 E-mail：mailto:rnabio@163.com Best regards, Carolyn Carolyn Miazga, PhD Scientific Support Supervisor Abcam Inc. www.abcam.com Abcam Customer Services and Scientific Support Team https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body [CCE3402261] Discover more at abcam.com

      Read More
      Answer

      Thank you for contacting Abcam. Regarding ab74538, when stored properly at -20, the antibody is expected to be stable for up to 5 years from receipt. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions

      Read More

      Question

      hello,Dear abcam company, hello! I have a production of antibodies when the problem is anti-Oct4antibody [EPR2054] (ab109183), hope to get answers, thank you very much!

      Read More
      Answer

      Thank you for contacting Abcam regarding ab109183. I am sorry to hear that you have been experiencing difficulties with this antibody. Can you please provide some additional details as to the nature of the problems you are experiencing and your protocol? I am happy to help you in any way that I can. I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

      Read More

      Question

      Which is the isotype of this antibody? In tha datasheet is reported to be IgG1 but this is a Rabbit antibody, not a mouse. Which secondary antibody do you suggest to use for WB analysis?

      Read More
      Answer

      Thank you for contacting Abcam about this. I have changed the datasheet to remedy the error that you have noticed. Ab109183 Anti-Oct4 antibody [EPR2054] is a rabbit IgG isotype. When using in Western Blot I would recommend using one of the following secondary antibodies: ab97051: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-ab97051.html or ab97064: Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-ab97064.html These products are covered by our Abpromise guarantee. At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund. The Abcam Abpromise® guarantee: • 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors. • We guarantee our products work in the tested species and applications stated on the datasheet. • We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. • We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards. Please note these conditions to our Abpromise®: • Protocol information must be provided in order for the claim to be validated. • Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples. • “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed. • Fast Track antibodies are covered for ELISA against the immunizing peptide only. I hope this information is helpful, but please do not hesitate to contact us with further questions.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com