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Synthetic peptide within Human Oct4 aa 250-350. The exact sequence is proprietary.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab109183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 39 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Blocking and diluting buffer: 5% NFDM/TBST.
Unpurified ab109183, at 1:500 dilution, staining Oct4 in paraffin-embedded Human ovarian dysgerminoma by Immunohistochemistry.
Unpurified ab109183 at 1/50 immunoprecipitating Oct4 in NCCIT (human pluripotent embryonal carcinoma) whole cell lysate observed at 39 KDa (lanes 1 and 2).
Lane 1 (input): NCCIT whole cell lysate, 10μg
Lane 2 (+): ab109183 + NCCIT whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109183 in NCCIT whole cell lysate
For western blotting, ab109183 at 1/1000 dilution and ab131366 VeriBlot for IP (HRP) was used as the secondary antibody at 1/10000.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Unpurified ab109183 showing negative staining in Colonic adenocarcinoma tissue.
Unpurified ab109183 showing negative staining in Skeletal muscle tissue.
Unpurified ab109183 showing negative staining in Normal liver tissue.
Unpurified ab109183 showing positive staining in Seminoma tissue.
Unpurified ab109183 showing negative staining in Normal brain tissue.
Unpurified ab109183 showing positive staining in Fetal brain tissue.
Unpurified ab109183 showing negative staining in Hepatocellular carcinoma tissue.
Unpurified ab109183 showing positive staining in Embryonal carcinoma tissue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"