Key features and details
- Rabbit polyclonal to Oct4, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Oct4 antibody, prediluted
See all Oct4 primary antibodies
DescriptionRabbit polyclonal to Oct4, prediluted
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Dog, Pig
Synthetic peptide within Human Oct4 aa 250-350 (internal sequence). The exact sequence is proprietary.
Database link: Q01860
- IHC-P: Human seminoma tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Ready to use.
Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
Incubate primary antibody for 30 minutes at room temperature.
FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Tissue specificityExpressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Sequence similaritiesBelongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
Developmental stageHighly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.
DomainThe POU-specific domain mediates interaction with PKM2.
modificationsSumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
Cellular localizationNucleus. Expressed in a diffuse and slightly punctuate pattern.
- Information by UniProt
- Octamer binding transcription factor 4 antibody
- MGC22487 antibody
- Oct 3 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab74536 has not yet been referenced specifically in any publications.