Overview

  • Product name

  • Description

    Rabbit polyclonal to Oct6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human Oct6 aa 187-221.
    Sequence:

    LHHALHEDGHEAQLEPSPPPHLGAHGHAHGHAHAG


    Database link: Q03052

  • Positive control

    • SK-MEL-30 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab220860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.
  • Tissue specificity

    Expressed in embryonal stem cells and in the developing brain.
  • Sequence similarities

    Belongs to the POU transcription factor family. Class-3 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • class 3 antibody
    • Oct-6 antibody
    • OCT6 antibody
    • Octamer-binding protein 6 antibody
    • Octamer-binding transcription factor 6 antibody
    • OTF-6 antibody
    • OTF6 antibody
    • PO3F1_HUMAN antibody
    • POU class 3 homeobox 1 antibody
    • POU domain antibody
    • POU domain class 3 transcription factor 1 antibody
    • POU domain transcription factor SCIP antibody
    • pou3f1 antibody
    • SCIP antibody
    • transcription factor 1 antibody
    see all

Images

  • Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized SK-MEL-30 cells labeling Oct6 with ab220860 at 4 µg/ml (green).

References

ab220860 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic spinal cord)
Permeabilization
Yes - 0.1%Triton X-100
Specification
Embryonic spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

Dr. Frederic Clotman

Verified customer

Submitted Aug 11 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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