Product nameAnti-Oct6 antibody
See all Oct6 primary antibodies
DescriptionRabbit polyclonal to Oct6
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Mouse Oct6 (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P21952
- WB: K562 nuclear extract. IHC-P: Mouse E10.5 embryo.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.
Tissue specificityExpressed in embryonal stem cells and in the developing brain.
Sequence similaritiesBelongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
- Information by UniProt
- class 3 antibody
- Oct-6 antibody
- OCT6 antibody
Paraffin-embedded mouse E10.5 embryo tissue stained for Oct6 using ab272924 at 1/500 dilution (green) in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
All lanes : Anti-Oct6 antibody (ab272924) at 1/500 dilution
Lane 1 : K562 whole cell extract
Lane 2 : K562 nuclear extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody
Paraffin-embedded mouse E10.5 embryo tissue stained for Oct6 using ab272924 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272924 has not yet been referenced specifically in any publications.