Key features and details
- Rabbit polyclonal to Oct6
- Suitable for: IHC-Fr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Oct6 antibody
See all Oct6 primary antibodies
DescriptionRabbit polyclonal to Oct6
Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Mouse Oct6. The exact sequence is proprietary. Center region.
Database link: P21952
- WB: Mouse fetal brain tissue extract. ICC/IF: A549 cells. IHC-P: Mouse E10.5 embryo tissue. IHC-Fr: Mouse cerebral cortex tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/100 - 1/1000.|
|WB||1/500 - 1/3000.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.
Tissue specificityExpressed in embryonal stem cells and in the developing brain.
Sequence similaritiesBelongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
- Information by UniProt
- class 3 antibody
- Oct-6 antibody
- OCT6 antibody
Paraffin-embedded mouse E10.5 embryo tissue stained for Oct6 using ab272925 at 1/500 dilution (green) in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes. Red: SOX2. Blue: DAPI.
Frozen mouse cerebral cortex tissue stained for Oct6 using ab272925 at 1/250 dilution (green) in immunohistochemical analysis. Blue: DAPI.
Anti-Oct6 antibody (ab272925) at 1/500 dilution + Mouse fetal brain tissue extract at 50 µg
HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
4% PFA-fixed A549 (Human lung carcinoma cell line) cells stained for Oct6 using ab272925 at 1/500 dilution in ICC/IF (green). Red: Phalloidin.
Paraffin-embedded mouse E10.5 embryo tissue stained for Oct6 using ab272925 at 1/250 dilution (green) in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
Red: Beta tubulin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272925 has not yet been referenced specifically in any publications.