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Neuroscience Cell Type Marker Neural Stem Cell marker
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RecombinantRabMAb

Recombinant Anti-Oct6 antibody [EP5421] (ab126746)

  • Datasheet
Reviews (1) Submit a question References (4)

Product price, shipping and contact information

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Find out more.

Western blot - Anti-Oct6 antibody [EP5421] (ab126746)
  • OI-RD Scanning - Anti-Oct6 antibody [EP5421] (ab126746)
  • Anti-Oct6 antibody [EP5421] (ab126746)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP5421] to Oct6
  • Suitable for: WB
  • Reacts with: Mouse, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Oct6 antibody [EP5421]
    See all Oct6 primary antibodies
  • Description

    Rabbit monoclonal [EP5421] to Oct6
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Oct6. The exact sequence is proprietary.

  • Positive control

    • F9 and NCCIT cell lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.87 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP5421
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • OCT
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • POU

Associated products

  • Alternative Versions

    • Anti-Oct6 antibody [EP5421] - BSA and Azide free (ab248163)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.
    • Tissue specificity

      Expressed in embryonal stem cells and in the developing brain.
    • Sequence similarities

      Belongs to the POU transcription factor family. Class-3 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q03052 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5453 Human
      • Entrez Gene: 18991 Mouse
      • Omim: 602479 Human
      • SwissProt: Q03052 Human
      • SwissProt: P21952 Mouse
      • Unigene: 1837 Human
      • Unigene: 297371 Mouse
      • Alternative names

        • class 3 antibody
        • Oct-6 antibody
        • OCT6 antibody
        • Octamer-binding protein 6 antibody
        • Octamer-binding transcription factor 6 antibody
        • OTF-6 antibody
        • OTF6 antibody
        • PO3F1_HUMAN antibody
        • POU class 3 homeobox 1 antibody
        • POU domain antibody
        • POU domain class 3 transcription factor 1 antibody
        • POU domain transcription factor SCIP antibody
        • pou3f1 antibody
        • SCIP antibody
        • transcription factor 1 antibody
        see all

      Images

      • Western blot - Anti-Oct6 antibody [EP5421] (ab126746)
        Western blot - Anti-Oct6 antibody [EP5421] (ab126746)
        All lanes : Anti-Oct6 antibody [EP5421] (ab126746) at 1/1000 dilution

        Lane 1 : F9 cell lysate
        Lane 2 : NCCIT cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 45 kDa
        Observed band size: 50 kDa
        why is the actual band size different from the predicted?

      • OI-RD Scanning - Anti-Oct6 antibody [EP5421] (ab126746)
        OI-RD Scanning - Anti-Oct6 antibody [EP5421] (ab126746)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-Oct6 antibody [EP5421] (ab126746)
        Anti-Oct6 antibody [EP5421] (ab126746)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (4)

      Publishing research using ab126746? Please let us know so that we can cite the reference in this datasheet.

      ab126746 has been referenced in 4 publications.

      • Grove M  et al. Axon-dependent expression of YAP/TAZ mediates Schwann cell remyelination but not proliferation after nerve injury. Elife 9:N/A (2020). PubMed: 32436841
      • Chaimowicz C  et al. Teashirt 1 (Tshz1) is essential for the development, survival and function of hypoglossal and phrenic motor neurons in mouse. Development 146:N/A (2019). PubMed: 31427287
      • Miyamoto Y  et al. BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by Schwann cells in mice. Sci Adv 4:eaar4471 (2018). PubMed: 29740613
      • Grove M  et al. YAP/TAZ initiate and maintain Schwann cell myelination. Elife 6:N/A (2017). PubMed: 28124973

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Oct6 antibody [EP5421]

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (E5.5 Mouse Embryo)
      Permeabilization
      Yes - 0.5% Triton X-100
      Specification
      E5.5 Mouse Embryo
      Blocking step
      Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Fixative
      Paraformaldehyde
      Read More

      Dr. Claire Simon

      Verified customer

      Submitted Jun 15 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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