Recombinant Anti-Oct6 antibody [EP5421] (ab126746)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Oct6 antibody [EP5421]
See all Oct6 primary antibodies -
Description
Rabbit monoclonal [EP5421] to Oct6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Oct6. The exact sequence is proprietary.
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Positive control
- F9 and NCCIT cell lysates
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.87 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5421 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab126746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis. -
Tissue specificity
Expressed in embryonal stem cells and in the developing brain. -
Sequence similarities
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5453 Human
- Entrez Gene: 18991 Mouse
- Omim: 602479 Human
- SwissProt: Q03052 Human
- SwissProt: P21952 Mouse
- Unigene: 1837 Human
- Unigene: 297371 Mouse
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Alternative names
- class 3 antibody
- Oct-6 antibody
- OCT6 antibody
see all
Images
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![Western blot - Anti-Oct6 antibody [EP5421] (ab126746)](https://www.abcam.com/ps/products/126/ab126746/Images/ab126746-140742-anti-oct6-antibody-ep5421-western-blot.jpg)
Western blot - Anti-Oct6 antibody [EP5421] (ab126746)All lanes : Anti-Oct6 antibody [EP5421] (ab126746) at 1/1000 dilution
Lane 1 : F9 cell lysate
Lane 2 : NCCIT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
![Other - Anti-Oct6 antibody [EP5421] (ab126746)](https://www.abcam.com/ps/products/126/ab126746/Images/ab126746-140741-anti-oct6-antibody-ep5421.jpg)
Other - Anti-Oct6 antibody [EP5421] (ab126746)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Miyamoto Y et al. BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by Schwann cells in mice. Sci Adv 4:eaar4471 (2018). Read more (PubMed: 29740613) »
- Grove M et al. YAP/TAZ initiate and maintain Schwann cell myelination. Elife 6:N/A (2017). Read more (PubMed: 28124973) »
