Overview

  • Product name
    Anti-Oct6 antibody [EP5421]
    See all Oct6 primary antibodies
  • Description
    Rabbit monoclonal [EP5421] to Oct6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Oct6. The exact sequence is proprietary.

  • Positive control
    • F9 and NCCIT cell lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.
    • Tissue specificity
      Expressed in embryonal stem cells and in the developing brain.
    • Sequence similarities
      Belongs to the POU transcription factor family. Class-3 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • class 3 antibody
      • Oct-6 antibody
      • OCT6 antibody
      • Octamer-binding protein 6 antibody
      • Octamer-binding transcription factor 6 antibody
      • OTF-6 antibody
      • OTF6 antibody
      • PO3F1_HUMAN antibody
      • POU class 3 homeobox 1 antibody
      • POU domain antibody
      • POU domain class 3 transcription factor 1 antibody
      • POU domain transcription factor SCIP antibody
      • pou3f1 antibody
      • SCIP antibody
      • transcription factor 1 antibody
      see all

    Images

    • All lanes : Anti-Oct6 antibody [EP5421] (ab126746) at 1/1000 dilution

      Lane 1 : F9 cell lysate
      Lane 2 : NCCIT cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 45 kDa
      Observed band size: 50 kDa (why is the actual band size different from the predicted?)

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (E5.5 Mouse Embryo)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    E5.5 Mouse Embryo
    Blocking step
    Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Claire Simon

    Verified customer

    Submitted Jun 15 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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