Recombinant Anti-Oct6 antibody [EP5421] (ab126746)
![Western blot - Anti-Oct6 antibody [EP5421] (ab126746)](https://www.abcam.com/ps/products/126/ab126746/Images/ab126746-140742-anti-oct6-antibody-ep5421-western-blot.jpg "Western blot - Anti-Oct6 antibody [EP5421] (ab126746)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP5421] to Oct6
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Oct6 antibody [EP5421]
See all Oct6 primary antibodies -
Description
Rabbit monoclonal [EP5421] to Oct6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide within Human Oct6. The exact sequence is proprietary.
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Positive control
- F9 and NCCIT cell lysates
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.87 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP5421 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis. -
Tissue specificity
Expressed in embryonal stem cells and in the developing brain. -
Sequence similarities
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5453 Human
- Entrez Gene: 18991 Mouse
- Omim: 602479 Human
- SwissProt: Q03052 Human
- SwissProt: P21952 Mouse
- Unigene: 1837 Human
- Unigene: 297371 Mouse
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Alternative names
- class 3 antibody
- Oct-6 antibody
- OCT6 antibody
see all
Images
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Western blot - Anti-Oct6 antibody [EP5421] (ab126746)All lanes : Anti-Oct6 antibody [EP5421] (ab126746) at 1/1000 dilution
Lane 1 : F9 cell lysate
Lane 2 : NCCIT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-Oct6 antibody [EP5421] (ab126746)](https://www.abcam.com/ps/products/126/ab126746/Images/ab126746-140741-anti-oct6-antibody-ep5421.jpg)
OI-RD Scanning - Anti-Oct6 antibody [EP5421] (ab126746) -
![Anti-Oct6 antibody [EP5421] (ab126746)](https://www.abcam.com/ps/products/126/ab126746/Images/ab126746-3-benefits-of-recombinant-antibodies.png)
Anti-Oct6 antibody [EP5421] (ab126746)
Datasheets and documents
References (4)
ab126746 has been referenced in 4 publications.
- Grove M et al. Axon-dependent expression of YAP/TAZ mediates Schwann cell remyelination but not proliferation after nerve injury. Elife 9:N/A (2020). PubMed: 32436841
- Chaimowicz C et al. Teashirt 1 (Tshz1) is essential for the development, survival and function of hypoglossal and phrenic motor neurons in mouse. Development 146:N/A (2019). PubMed: 31427287
- Miyamoto Y et al. BIG1/Arfgef1 and Arf1 regulate the initiation of myelination by Schwann cells in mice. Sci Adv 4:eaar4471 (2018). PubMed: 29740613
- Grove M et al. YAP/TAZ initiate and maintain Schwann cell myelination. Elife 6:N/A (2017). PubMed: 28124973
