OcTMAB™, dynamin I and dynamin II inhibitor (ab120467)
Key features and details
- Cell-permeable dynamin I and dynamin II inhibitor
- CAS Number: 1120-02-1
- Soluble in DMSO to 25 mM (with warming)
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
OcTMAB™, dynamin I and dynamin II inhibitor -
Description
Cell-permeable dynamin I and dynamin II inhibitor -
Biological description
Cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 1.9 and 4.4 μM for inhibition of dynamin I and II GTPase, respectively). Targets the pleckstrin homology (PH) (lipid binding) domain. Competitive with phospholipid and non-competitive with GTP. Inhibits receptor-mediated endocytosis (IC50 = 6.7 μM). Inhibits cancer cell growth.
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General notes
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. OcTMAB™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd. -
CAS Number
1120-02-1 -
Chemical structure
Properties
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Chemical name
Octadecyltrimethylammonium bromide -
Molecular weight
392.51 -
Molecular formula
C21H46BrN -
PubChem identifier
70708 -
Storage instructions
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 25 mM (with warming) -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[Br-].C[N+](C)(C)CCCCCCCCCCCCCCCCCC -
Source
Synthetic
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - OcTMAB™, dynamin I and dynamin II inhibitor (ab120467)2D chemical structure image of ab120467, OcTMAB™, dynamin I and dynamin II inhibitor
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Functional Studies - OcTMAB™, dynamin I and dynamin II inhibitor (ab120467)ab66705 staining PAI1 in HeLa cells treated with OcTMAB™ (ab120467), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of OcTMAB™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120467 (OcTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab120467 has been referenced in 1 publication.
- Wang D et al. ERK is involved in the differentiation and function of dimethyl sulfoxide-induced HL-60 neutrophil-like cells, which mimic inflammatory neutrophils. Int Immunopharmacol 84:106510 (2020). PubMed: 32361568