Overview

  • Product name

  • Description

    Rabbit polyclonal to ODF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human ODF1 aa 88-160.
    Sequence:

    RSLERKAIRAIEDEKRELAKLRRTTNRILASSCCSSNILGSVNVCGFEPD QVKVRVKDGKVCVSAERENRYDC


    Database link: Q14990

  • Positive control

    • Human testis tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab204577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the outer dense fibers (ODF) of spermatozoa. ODF are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail.
  • Tissue specificity

    Testis.
  • Domain

    The C-terminal contains many C-X-P repeats.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cancer/testis antigen 133 antibody
    • CT133 antibody
    • HSPB10 antibody
    • ODF antibody
    • Odf1 antibody
    • ODF2 antibody
    • ODF27 antibody
    • ODFP antibody
    • ODFP1_HUMAN antibody
    • ODFPG antibody
    • ODFPGA antibody
    • ODFPGB antibody
    • Outer dense fiber of sperm tails 1 antibody
    • Outer dense fiber of sperm tails, 27 kD antibody
    • Outer dense fiber protein 1 antibody
    • RT7 antibody
    • SODF antibody
    see all

Images

  • Immunohistochemical staining of Human testis tissue labeling ODF1 using ab204577 at 1/500 dilution.

References

This product has been referenced in:

  • He Y  et al. Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2†. Biol Reprod 100:561-572 (2019). Read more (PubMed: 30295753) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate-based buffer, pH6
Permeabilization
No
Specification
Mouse testis
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 26 2018

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