Recombinant Anti-ODF1 antibody [EPR15496] (ab197029)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ODF1 antibody [EPR15496]
See all ODF1 primary antibodies -
Description
Rabbit monoclonal [EPR15496] to ODF1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Mouse ODF1 aa 1-100. The exact sequence is proprietary.
Database link: Q61999 -
Positive control
- WB: Mouse testis lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15496 -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab197029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/20000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
Target
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Function
Component of the outer dense fibers (ODF) of spermatozoa. ODF are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. -
Tissue specificity
Testis. -
Domain
The C-terminal contains many C-X-P repeats. - Information by UniProt
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Database links
- Entrez Gene: 18285 Mouse
- SwissProt: Q61999 Mouse
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Alternative names
- Cancer/testis antigen 133 antibody
- CT133 antibody
- HSPB10 antibody
see all
Images
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![Western blot - Anti-ODF1 antibody [EPR15496] (ab197029)](https://www.abcam.com/ps/products/197/ab197029/Images/ab197029-236824-anti-odf1-antibody-epr15496-western-blot.jpg)
Western blot - Anti-ODF1 antibody [EPR15496] (ab197029)All lanes : Anti-ODF1 antibody [EPR15496] (ab197029) at 1/20000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Protocols
Datasheets and documents
References
ab197029 has not yet been referenced specifically in any publications.
