Anti-OFD1 antibody (ab222837)
Key features and details
- Rabbit polyclonal to OFD1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OFD1 antibody -
Description
Rabbit polyclonal to OFD1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human OFD1 aa 550-800.
Database link: O75665 -
Positive control
- WB: Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysate. IHC-P: Human adrenal gland and skeletal muscle tissues. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
>95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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WB |
1/500 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 43,112,117 kDa).
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Notes |
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IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 43,112,117 kDa). |
Target
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Function
Component of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis. Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation. -
Tissue specificity
Widely expressed. Expressed in 9 and 14 weeks old embryos in metanephric mesenchyme, oral mucosa, lung, heart, nasal and cranial cartilage, and brain. Expressed in metanephros, brain, tongue, and limb. -
Involvement in disease
Orofaciodigital syndrome 1
Simpson-Golabi-Behmel syndrome 2
Joubert syndrome 10
Retinitis pigmentosa 23 -
Sequence similarities
Belongs to the OFD1 family.
Contains 1 LisH domain. -
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, cilium basal body. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Localizes to centriole distal ends and to centriolar satellites (PubMed:20230748, PubMed:24121310). - Information by UniProt
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Database links
- Entrez Gene: 8481 Human
- Omim: 300170 Human
- SwissProt: O75665 Human
- Unigene: 6483 Human
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Alternative names
- 71 7A antibody
- 717A antibody
- CXorf5 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labelling OFD1 with ab222837 at 1/100 dilution, followed by a Goat anti-Mouse IgG antibody labelled by HRP and visualised using 0.05% DAB.
Antigen retrieval was performed using high pressure in a citrate buffer, pH 6.0. Section was blocked with 10% normal goat serum for 30 minutes at 37°C, before being incubated with the primary antibody at 4°C overnight.
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Lane 1: Hela whole cell lysate.
Lane 2: SH-SY5Y whole cell lysate.
Lane 3: K562 whole cell lysate.
All lanes: ab222837 antibody at 1:2000 dilution.
Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 117, 43, 112 kDa Observed band size: 117 kDa.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for OFD1 (green) using ab222837 at 1/100 dilution in ICC/IF. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) was used as secondary antibody.
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Paraffin-embedded human adrenal gland tissue stained for OFD1 using ab222837 at 1/100 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab222837 has been referenced in 4 publications.
- Liu P et al. NRF2 negatively regulates primary ciliogenesis and hedgehog signaling. PLoS Biol 18:e3000620 (2020). PubMed: 32053600
- Saralamma VVG et al. Comparative proteomic analysis uncovers potential biomarkers involved in the anticancer effect of Scutellarein in human gastric cancer cells. Oncol Rep 44:939-958 (2020). PubMed: 32705238
- Kyun ML et al. Wnt3a Stimulation Promotes Primary Ciliogenesis through ß-Catenin Phosphorylation-Induced Reorganization of Centriolar Satellites. Cell Rep 30:1447-1462.e5 (2020). PubMed: 32023461
- Hsiao CJ et al. Gli2 modulates cell cycle re-entry through autophagy-mediated regulation of the length of primary cilia. J Cell Sci 131:N/A (2018). PubMed: 30463852