Key features and details
- Rabbit polyclonal to OFD1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-OFD1 antibody
DescriptionRabbit polyclonal to OFD1
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse skeletal muscle tissue lysate. IHC-P: Human adrenal gland tissue. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab222837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 43,112,117 kDa).|
FunctionComponent of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis. Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation.
Tissue specificityWidely expressed. Expressed in 9 and 14 weeks old embryos in metanephric mesenchyme, oral mucosa, lung, heart, nasal and cranial cartilage, and brain. Expressed in metanephros, brain, tongue, and limb.
Involvement in diseaseOrofaciodigital syndrome 1
Simpson-Golabi-Behmel syndrome 2
Joubert syndrome 10
Retinitis pigmentosa 23
Sequence similaritiesBelongs to the OFD1 family.
Contains 1 LisH domain.
Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, cilium basal body. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Localizes to centriole distal ends and to centriolar satellites (PubMed:20230748, PubMed:24121310).
- Information by UniProt
- 71 7A antibody
- 717A antibody
- CXorf5 antibody
Anti-OFD1 antibody (ab222837) at 1/500 dilution + Mouse skeletal muscle tissue lysate
Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 43,112,117 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted?
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for OFD1 (green) using ab222837 at 1/100 dilution in ICC/IF. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) was used as secondary antibody.
Paraffin-embedded human adrenal gland tissue stained for OFD1 using ab222837 at 1/100 dilution in immunohistochemical analysis.
ab222837 has been referenced in 3 publications.
- Liu P et al. NRF2 negatively regulates primary ciliogenesis and hedgehog signaling. PLoS Biol 18:e3000620 (2020). PubMed: 32053600
- Saralamma VVG et al. Comparative proteomic analysis uncovers potential biomarkers involved in the anticancer effect of Scutellarein in human gastric cancer cells. Oncol Rep 44:939-958 (2020). PubMed: 32705238
- Hsiao CJ et al. Gli2 modulates cell cycle re-entry through autophagy-mediated regulation of the length of primary cilia. J Cell Sci 131:N/A (2018). PubMed: 30463852