Overview

  • Product name

  • Description

    Rabbit polyclonal to OGDH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 2 - 51 (FHLRTCAAKL RPLTASQTVK TFSQNRPAAA RTFQQIRCYS APVAAEPFLS) of Human OGDH (NP_001003941)

  • Positive control

    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
  • Sequence similarities

    Belongs to the alpha-ketoglutarate dehydrogenase family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2 oxoglutarate dehydrogenase antibody
    • 2 oxoglutarate dehydrogenase complex component E1 antibody
    • 2 oxoglutarate dehydrogenase mitochondrial antibody
    • 2-oxoglutarate dehydrogenase antibody
    • 2-oxoglutarate dehydrogenase complex component E1 antibody
    • AKGDH antibody
    • Alpha ketoglutarate dehydrogenase antibody
    • Alpha-ketoglutarate dehydrogenase antibody
    • E1k antibody
    • mitochondrial antibody
    • ODO1_HUMAN antibody
    • OGDC antibody
    • OGDC E1 antibody
    • OGDC-E1 antibody
    • OGDH antibody
    • Oxoglutarate (alpha ketoglutarate) dehydrogenase (lipoamide) antibody
    • Oxoglutarate decarboxylase antibody
    • Oxoglutarate dehydrogenase (lipoamide) antibody
    • Oxoglutarate dehydrogenase (succinyl transferring) antibody
    see all

Images

  • Anti-OGDH antibody (ab87057) at 1 µg/ml + Human fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 48 kDa

References

This product has been referenced in:

  • Yang W  et al. Mitochondrial Sirtuin Network Reveals Dynamic SIRT3-Dependent Deacetylation in Response to Membrane Depolarization. Cell 167:985-1000.e21 (2016). Read more (PubMed: 27881304) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Raw 264.7 cells and HepG2 cells)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Raw 264.7 cells and HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Feb 16 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Specification
Prostate cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jul 04 2016

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