Anti-Ogg1 antibody (ab233214)
Key features and details
- Rabbit polyclonal to Ogg1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Ogg1 antibody
See all Ogg1 primary antibodies -
Description
Rabbit polyclonal to Ogg1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Ogg1 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: O15527 -
Positive control
- WB: Pig heart and kidney lysate; Recombinant human Ogg1 protein. IHC-P: Human brain and liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 39 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 39 kDa. |
Target
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Function
DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. -
Sequence similarities
Belongs to the type-1 OGG1 family. -
Cellular localization
Mitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells. - Information by UniProt
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Database links
- Entrez Gene: 4968 Human
- Entrez Gene: 100155036 Pig
- Omim: 601982 Human
- SwissProt: O15527 Human
- Unigene: 380271 Human
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Alternative names
- 8 hydroxyguanine DNA glycosylase antibody
- 8 oxoguanine DNA glycosylase 1 antibody
- 8 oxoguanine DNA glycosylase antibody
see all
Images
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Formalin-fixed, paraffin-embedded human liver tissue stained for Ogg1 using ab233214 at 30 µg/mL in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human brain tissue stained for Ogg1 using ab233214 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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All lanes : Anti-Ogg1 antibody (ab233214) at 2 µg/ml
Lane 1 : Pig heart lysate
Lane 2 : Pig kidney lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 39 kDa -
Anti-Ogg1 antibody (ab233214) at 2 µg/ml + Recombinant human Ogg1 protein
Predicted band size: 39 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233214 has not yet been referenced specifically in any publications.