Key features and details
- Rabbit polyclonal to Ogg1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-Ogg1 antibody
See all Ogg1 primary antibodies
DescriptionRabbit polyclonal to Ogg1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Recombinant fragment (His-T7-tag) corresponding to Human Ogg1 aa 31-345. (Expressed in E.coli).
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Database link: O15527
- WB: Pig heart and kidney lysate; Recombinant human Ogg1 protein. IHC-P: Human brain and liver tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 39 kDa.|
FunctionDNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
Involvement in diseaseDefects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Sequence similaritiesBelongs to the type-1 OGG1 family.
Cellular localizationMitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells.
- Information by UniProt
- 8 hydroxyguanine DNA glycosylase antibody
- 8 oxoguanine DNA glycosylase 1 antibody
- 8 oxoguanine DNA glycosylase antibody
Formalin-fixed, paraffin-embedded human liver tissue stained for Ogg1 using ab233214 at 30 µg/mL in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human brain tissue stained for Ogg1 using ab233214 at 20 µg/mL in immunohistochemical analysis. DAB staining.
All lanes : Anti-Ogg1 antibody (ab233214) at 2 µg/ml
Lane 1 : Pig heart lysate
Lane 2 : Pig kidney lysate
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 39 kDa
Anti-Ogg1 antibody (ab233214) at 2 µg/ml + Recombinant human Ogg1 protein
Predicted band size: 39 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233214 has not yet been referenced specifically in any publications.