Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)
Key features and details
- Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies -
Description
Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Cow, Pig
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Immunogen
Recombinant fragment corresponding to Human OGT/ O-Linked N-Acetylglucosamine Transferase aa 213-462.
Database link: O15294 -
Positive control
- WB: HeLa, PC-12 cells. Mouse brain tissue extracts. ICC/IF: MCF7, DU145, HeLa cells. IHC-P: Cal27 Xenograft. Rat hind brain. IP: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| WB | (8) |
1/500 - 1/3000. Predicted molecular weight: 117 kDa.
|
| IHC-P | (1) |
1/100 - 1/1000.
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| IP |
1/100 - 1/500.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 117 kDa. |
|
IHC-P
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
Target
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Function
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
Tissue specificity
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
Domain
The TPR repeat domain mediates recognition of protein substrates. -
Post-translational
modificationsUbiquitinated, leading to its proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 532053 Cow
- Entrez Gene: 8473 Human
- Entrez Gene: 108155 Mouse
- Entrez Gene: 664652 Pig
- Entrez Gene: 26295 Rat
- Entrez Gene: 337685 Zebrafish
- Omim: 300255 Human
- SwissProt: O15294 Human
see all -
Alternative names
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
Images
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 117 kDa
7.5% SDS PAGE -
Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 5 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)HeLa cells stained for O-GlcNAc (green) using ab96718 at 1/200 dilution in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using ab96718 at 1/100 dilution. -
Immunoprecipitation - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Immunoprecipitation analysis of OGT/O-linked N-Acetylglucosamine Transferase protein from A431 whole cell extracts using ab96718 (5µg). Western blot analysis was performed using ab96718 and an Easyblot anti-rabbit IgG was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Ab96718 staining OGT / O-Linked N-Acetylglucosamine Transferase in MCF7 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). MFC7 cells were fixed with paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody at a 1:500 dilution.
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + PC-12 whole cell extract at 30 µg
Predicted band size: 117 kDa5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)ICC/IF image of ab96718 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96718, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Paraffin embedded rat hind brain tissue stained for O-GlcNAc using ab96718 at 1/500 in immunohistochemical analysis.
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718)Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/2000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 117 kDa7.5% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab96718 has been referenced in 27 publications.
- Shang M et al. The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation. Nat Commun 12:1940 (2021). PubMed: 33782411
- Qian L et al. Reduced O-GlcNAcylation of SNAP-23 promotes cisplatin resistance by inducing exosome secretion in ovarian cancer. Cell Death Discov 7:112 (2021). PubMed: 34001861
- Xu TH et al. OGT-Mediated KEAP1 Glycosylation Accelerates NRF2 Degradation Leading to High Phosphate-Induced Vascular Calcification in Chronic Kidney Disease. Front Physiol 11:1092 (2020). PubMed: 33192538
- Paredes F et al. Mitochondrial Protein Poldip2 (Polymerase Delta Interacting Protein 2) Controls Vascular Smooth Muscle Differentiated Phenotype by O-Linked GlcNAc (N-Acetylglucosamine) Transferase-Dependent Inhibition of a Ubiquitin Proteasome System. Circ Res 126:41-56 (2020). PubMed: 31656131
- Yang Y et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761