Product nameAnti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies
DescriptionRabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Zebrafish
Synthetic peptide: MASSVGNVADSTEPTKRMLSFQGLAELAHREYQAGDFEAAERHCMQLWRQ EPDNTGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYK ERGQLQEAIEHYRHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSAL QYNPDLYCVRSDLGNLLKALGRLEEAKACYLKAIETQPNFAVAWSNLGCV FNAQGEIWLAIHHFEKAVTLDPNFLD AYINLGNVLKEARI, corresponding to amino acids 1 - 240 of Human O-Linked N-Acetylglucosamine Transferase (NP_858059).
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates This antibody gave a positive result when used in the following methanol fixed cell lines: DU145.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa nuclear extract lysate (ab14655)
- HepG2 cytoplasmic extract lysate (ab14659)
- HepG2 nuclear extract lysate (ab14660)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- A431 whole cell lysate (ab30132)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- MOLT4 whole cell lysate (ab7912)
Our Abpromise guarantee covers the use of ab97274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/3000. Predicted molecular weight: 117 kDa.|
FunctionAddition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Tissue specificityHighly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats.
DomainThe TPR repeat domain mediates recognition of protein substrates.
modificationsUbiquitinated, leading to its proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Mostly in the nucleus.
- Information by UniProt
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
ICC/IF image of ab97274 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab97274 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab97274) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 117 kDa
7.5% SDS PAGE
ab97274 has not yet been referenced specifically in any publications.