RecombinantRabMAb

Recombinant Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Flow Cytometry - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]
    See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies

  • Description

    Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human OGT/ O-Linked N-Acetylglucosamine Transferase aa 1000 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O15294

  • Positive control

    • WB: A549, HeLa, U87-MG and HEK-293T cell lysates. IHC-P: Human pancreas tissue. Flow Cyt: HEK-293T cells. ICC/IF: K562 and A549 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (2)
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).
ICC/IF
1/50 - 1/100.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).
ICC/IF
1/50 - 1/100.
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.

    • Tissue specificity

      Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

    • Pathway

      Protein modification; protein glycosylation.

    • Sequence similarities

      Belongs to the O-GlcNAc transferase family.
      Contains 13 TPR repeats.

    • Domain

      The TPR repeat domain mediates recognition of protein substrates.

    • Post-translational
      modifications

      Ubiquitinated, leading to its proteasomal degradation.

    • Cellular localization

      Cytoplasm. Nucleus. Mostly in the nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • FLJ23071 antibody
      • GlcNAc transferase antibody
      • HRNT1 antibody
      • MGC22921 antibody
      • O GlcNAc antibody
      • O GlcNAc transferase p110 subunit antibody
      • O GlcNAc transferase subunit p110 antibody
      • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
      • O linked N acetylglucosamine (GlcNAc) transferase antibody
      • O linked N acetylglucosamine transferase 110 kDa subunit antibody
      • O-GlcNAc transferase subunit p110 antibody
      • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
      • ogt antibody
      • OGT1_HUMAN antibody
      • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
      • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
      • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
      • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
      • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
      see all

    Images

    • Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
      Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
      All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : U87-MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 116 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
      Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

      ab177941 staining OGT / O-Linked N-Acetylglucosamine Transferase antibody in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

      Negative control 1: PBS only.

    • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
      Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

      Immunofluorescent analysis of A549 cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
      Flow Cytometry - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

      Flow cytometric analysis of permeabilized 293T cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/10 dilution (red)  or a rabbit IgG (negative) (green).

    • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
      Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

    References (13)

    Publishing research using ab177941? Please let us know so that we can cite the reference in this datasheet.

    ab177941 has been referenced in 13 publications.

    • Huang R  et al. Increased Ratio of Global O-GlcNAcylation to Tau Phosphorylation at Thr212 Site Is Associated With Better Memory Function in Patients With Type 2 Diabetes. Front Physiol 10:110 (2019). PubMed: 30837891
    • Munemoto M  et al. Roles of the hexosamine biosynthetic pathway and pentose phosphate pathway in bile acid-induced cancer development. Cancer Sci 110:2408-2420 (2019). PubMed: 31215094
    • Chen Y  et al. O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer. Cell Signal 63:109384 (2019). PubMed: 31394193
    • Hao Y  et al. Next-generation unnatural monosaccharides reveal that ESRRB O-GlcNAcylation regulates pluripotency of mouse embryonic stem cells. Nat Commun 10:4065 (2019). PubMed: 31492838
    • Sanin DE  et al. Mitochondrial Membrane Potential Regulates Nuclear Gene Expression in Macrophages Exposed to Prostaglandin E2. Immunity 49:1021-1033.e6 (2018). PubMed: 30566880
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Gastric Cancer Cell Line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    5 µg
    Specification
    Gastric Cancer Cell Line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 01 2020

    Western blot abreview for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse macrophage cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    5 µg
    Specification
    Mouse macrophage cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 01 2020

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