RecombinantRabMAb

Recombinant Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Flow Cytometry (Intracellular) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
  • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]
    See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies

  • Description

    Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, HeLa, U87-MG and HEK-293T cell lysates. IHC-P: Human pancreas tissue. Flow Cyt (intra): HEK-293T cells. ICC/IF: K562 and A549 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (2)
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).
ICC/IF
1/50 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).
ICC/IF
1/50 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Function

    Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.

  • Tissue specificity

    Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

  • Pathway

    Protein modification; protein glycosylation.

  • Sequence similarities

    Belongs to the O-GlcNAc transferase family.
    Contains 13 TPR repeats.

  • Domain

    The TPR repeat domain mediates recognition of protein substrates.

  • Post-translational
    modifications

    Ubiquitinated, leading to its proteasomal degradation.

  • Cellular localization

    Cytoplasm. Nucleus. Mostly in the nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ23071 antibody
    • GlcNAc transferase antibody
    • HRNT1 antibody
    • MGC22921 antibody
    • O GlcNAc antibody
    • O GlcNAc transferase p110 subunit antibody
    • O GlcNAc transferase subunit p110 antibody
    • O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) antibody
    • O linked N acetylglucosamine (GlcNAc) transferase antibody
    • O linked N acetylglucosamine transferase 110 kDa subunit antibody
    • O-GlcNAc transferase subunit p110 antibody
    • O-linked N-acetylglucosamine transferase 110 kDa subunit antibody
    • ogt antibody
    • OGT1_HUMAN antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP N acetylglucosamine peptide N acetylglucosaminyltransferase GlcNAc transferase antibody
    • UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody
    • UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase antibody
    • Uridinediphospho N acetylglucosamine:polypeptide beta N acetylglucosaminyl transferase antibody
    see all

Images

  • Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 116 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

    ab177941 staining OGT / O-Linked N-Acetylglucosamine Transferase antibody in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

  • Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

    Immunofluorescent analysis of A549 cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/50 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Flow Cytometry (Intracellular) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

    Intracellular flow cytometric analysis of permeabilized 293T cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/10 dilution (red) or a rabbit IgG (negative) (green). 

     

     

  • Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
    Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)

References (18)

Publishing research using ab177941? Please let us know so that we can cite the reference in this datasheet.

ab177941 has been referenced in 18 publications.

  • Zhu G  et al. O-GlcNAcylation enhances sensitivity to RSL3-induced ferroptosis via the YAP/TFRC pathway in liver cancer. Cell Death Discov 7:83 (2021). PubMed: 33863873
  • Oliveira-Nunes MC  et al. O-GlcNAcylation protein disruption by Thiamet G promotes changes on the GBM U87-MG cells secretome molecular signature. Clin Proteomics 18:14 (2021). PubMed: 33902430
  • Li C  et al. Hypertonic stress modulates eNOS function through O-GlcNAc modification at Thr-866. Sci Rep 11:11272 (2021). PubMed: 34050207
  • Xu TH  et al. OGT-Mediated KEAP1 Glycosylation Accelerates NRF2 Degradation Leading to High Phosphate-Induced Vascular Calcification in Chronic Kidney Disease. Front Physiol 11:1092 (2020). PubMed: 33192538
  • Munemoto M  et al. Roles of the hexosamine biosynthetic pathway and pentose phosphate pathway in bile acid-induced cancer development. Cancer Sci 110:2408-2420 (2019). PubMed: 31215094
View all Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Gastric Cancer Cell Line)
Gel Running Conditions
Reduced Denaturing
Loading amount
5 µg
Specification
Gastric Cancer Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 01 2020

Western blot abreview for Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse macrophage cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
5 µg
Specification
Mouse macrophage cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Dec 01 2020

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