Recombinant Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
![Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.com/ps/products/177/ab177941/Images/ab177941-205953-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-western-blot.jpg "Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]
See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies -
Description
Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, HeLa, U87-MG and HEK-293T cell lysates. IHC-P: Human pancreas tissue. Flow Cyt (intra): HEK-293T cells. ICC/IF: K562 and A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12713 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab198532)
- Alexa Fluor® 555 Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab215809)
- Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] - BSA and Azide free (ab236014)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (2) |
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).
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| ICC/IF |
1/50 - 1/100.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa). |
|
ICC/IF
1/50 - 1/100. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
Tissue specificity
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
Domain
The TPR repeat domain mediates recognition of protein substrates. -
Post-translational
modificationsUbiquitinated, leading to its proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8473 Human
- Entrez Gene: 108155 Mouse
- Entrez Gene: 26295 Rat
- Omim: 300255 Human
- SwissProt: O15294 Human
- SwissProt: Q8CGY8 Mouse
- SwissProt: P56558 Rat
- Unigene: 405410 Human
see all -
Alternative names
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
Images
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Western blot - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 116 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.com/ps/products/177/ab177941/Images/ab177941-264992-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)ab177941 staining OGT / O-Linked N-Acetylglucosamine Transferase antibody in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control 1: PBS only.
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![Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.com/ps/products/177/ab177941/Images/ab177941-205955-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)Immunofluorescent analysis of A549 cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.com/ps/products/177/ab177941/Images/ab177941-205954-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.com/ps/products/177/ab177941/Images/ab177941-205957-anti-ogt-o-linked-n-acetylglucosamine-transferase-antibody-epr12713-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)Intracellular flow cytometric analysis of permeabilized 293T cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)](https://www.abcam.com/ps/products/177/ab177941/Images/ab177941-6-benefits-of-recombinant-antibodies.png)
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941)
Protocols
Datasheets and documents
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Datasheet download
References (18)
ab177941 has been referenced in 18 publications.
- Zhu G et al. O-GlcNAcylation enhances sensitivity to RSL3-induced ferroptosis via the YAP/TFRC pathway in liver cancer. Cell Death Discov 7:83 (2021). PubMed: 33863873
- Oliveira-Nunes MC et al. O-GlcNAcylation protein disruption by Thiamet G promotes changes on the GBM U87-MG cells secretome molecular signature. Clin Proteomics 18:14 (2021). PubMed: 33902430
- Li C et al. Hypertonic stress modulates eNOS function through O-GlcNAc modification at Thr-866. Sci Rep 11:11272 (2021). PubMed: 34050207
- Xu TH et al. OGT-Mediated KEAP1 Glycosylation Accelerates NRF2 Degradation Leading to High Phosphate-Induced Vascular Calcification in Chronic Kidney Disease. Front Physiol 11:1092 (2020). PubMed: 33192538
- Munemoto M et al. Roles of the hexosamine biosynthetic pathway and pentose phosphate pathway in bile acid-induced cancer development. Cancer Sci 110:2408-2420 (2019). PubMed: 31215094
