Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135)
Key features and details
- Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies -
Description
Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase -
Host species
Goat -
Specificity
ab59135 is expected to recognize both reported isoforms of O-Linked N-Acetylglucosamine -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Cow, Dog, Pig, Xenopus laevis, Zebrafish -
Immunogen
Synthetic peptide:
C-YEHPKDLKLSDGR
by a Cysteine residue linker, corresponding to amino acids 543-555 of Human O-Linked N-Acetylglucosamine -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.
A 1 hour primary incubation is recommended for this product. |
Notes |
---|
WB
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa. A 1 hour primary incubation is recommended for this product. |
Target
-
Function
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
Tissue specificity
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
Domain
The TPR repeat domain mediates recognition of protein substrates. -
Post-translational
modificationsUbiquitinated, leading to its proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 532053 Cow
- Entrez Gene: 108155 Mouse
- Entrez Gene: 664652 Pig
- Entrez Gene: 26295 Rat
- Entrez Gene: 337685 Zebrafish
- SwissProt: Q8CGY8 Mouse
- SwissProt: Q27HV0 Pig
- SwissProt: P56558 Rat
see all -
Alternative names
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
Images
-
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135) at 0.05 µg/ml + Rat pancreas lysate in RIPA buffer at 35 µg
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Preliminary testing was unsuccessful on Mouse Brain for this particular batch.
Datasheets and documents
-
Datasheet download
References (3)
ab59135 has been referenced in 3 publications.
- Moura CS et al. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. Food Nutr Res 61:1290740 (2017). WB ; Rat . PubMed: 28326005
- Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . PubMed: 24465380
- Andrés-Bergós J et al. The increase in o-linked N-acetylglucosamine protein modification stimulates chondrogenic differentiation both in vitro and in vivo. J Biol Chem 287:33615-28 (2012). WB . PubMed: 22859309