Key features and details
- Goat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies
DescriptionGoat polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
Specificityab59135 is expected to recognize both reported isoforms of O-Linked N-Acetylglucosamine
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Cow, Dog, Pig, Xenopus laevis, Zebrafish
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab59135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 110 kDa.
A 1 hour primary incubation is recommended for this product.
FunctionAddition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Tissue specificityHighly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats.
DomainThe TPR repeat domain mediates recognition of protein substrates.
modificationsUbiquitinated, leading to its proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Mostly in the nucleus.
- Information by UniProt
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab59135) at 0.05 µg/ml + Rat pancreas lysate in RIPA buffer at 35 µg
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Preliminary testing was unsuccessful on Mouse Brain for this particular batch.
ab59135 has been referenced in 3 publications.
- Moura CS et al. Bioactivity of food peptides: biological response of rats to bovine milk whey peptides following acute exercise. Food Nutr Res 61:1290740 (2017). WB ; Rat . PubMed: 28326005
- Moura CS et al. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS One 9:e83437 (2014). WB ; Rat . PubMed: 24465380
- Andrés-Bergós J et al. The increase in o-linked N-acetylglucosamine protein modification stimulates chondrogenic differentiation both in vitro and in vivo. J Biol Chem 287:33615-28 (2012). WB . PubMed: 22859309