Product nameAnti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
See all OGT / O-Linked N-Acetylglucosamine Transferase primary antibodies
DescriptionRabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
Tested applicationsSuitable for: WB, IHC-P, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Zebrafish
Predicted to work with: Rat, Cow, Pig
Recombinant protein fragment corresponding to a region within amino acids 213 and 462 of O-Linked N-Acetylglucosamine Transferase
- 293T, H1299, HeLa, Molt-4 and Raji cell lysates. Cal27 xenograft. IF: DU145 cell line
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.025% CMIT/MIT 3:1
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 117 kDa.|
|IHC-P||1/100 - 1/250.|
|IP||1/100 - 1/500.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionAddition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1.
Tissue specificityHighly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats.
DomainThe TPR repeat domain mediates recognition of protein substrates.
modificationsUbiquitinated, leading to its proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Mostly in the nucleus.
- Information by UniProt
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 117 kDa
7.5% SDS PAGE
All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 5 minutes
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using ab96718 at 1/100 dilution.
Immunoprecipitation analysis of OGT/O-linked N-Acetylglucosamine Transferase protein from A431 whole cell extracts using ab96718 (5µg). Western blot analysis was performed using ab96718 and an Easyblot anti-rabbit IgG was used as the secondary antibody.
Ab96718 staining OGT / O-Linked N-Acetylglucosamine Transferase in MCF7 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). MFC7 cells were fixed with paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody at a 1:500 dilution.
ICC/IF image of ab96718 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96718, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Lo WY et al. MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression. Front Physiol 9:355 (2018). Read more (PubMed: 29720943) »
- Pyo KE et al. ULK1 O-GlcNAcylation Is Crucial for Activating VPS34 via ATG14L during Autophagy Initiation. Cell Rep 25:2878-2890.e4 (2018). Read more (PubMed: 30517873) »