Overview

  • Product name

    Anti-OIF antibody - C-terminal
    See all OIF primary antibodies
  • Description

    Rabbit polyclonal to OIF - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human OIF aa 246-276 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P20774

  • Positive control

    • CEM cell line lysate; Human lung pleura tissue; 293 cell lysate transiently transfected with OIF; 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab211456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/100. Predicted molecular weight: 34 kDa.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2.
  • Tissue specificity

    Bone.
  • Sequence similarities

    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class III subfamily.
    Contains 7 LRR (leucine-rich) repeats.
  • Post-translational
    modifications

    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Corneal keratan sulfate proteoglycan antibody
    • DKFZP586P2421 antibody
    • MIME_HUMAN antibody
    • Mimecan antibody
    • Mimecan proteoglycan antibody
    • OG antibody
    • OGN antibody
    • OIF antibody
    • Osteoglycin antibody
    • Osteoinductive factor antibody
    • SLRR3A antibody
    see all

Images

  • Anti-OIF antibody - C-terminal (ab211456) at 1/1000 dilution + CEM cell line lysate at 35 µg

    Predicted band size: 34 kDa

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human lung pleura tissue labeling OIF with ab211456 at 1/100 dilution.

  • Flow Cytometrical analysis of 293 cells labeling OIF with ab211456 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • All lanes : Anti-OIF antibody - C-terminal (ab211456) at 1/1000 dilution

    Lane 1 : 293 cell lysate non-transfected
    Lane 2 : 293 cell lysate transiently transfected with OIF

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 34 kDa

References

ab211456 has not yet been referenced specifically in any publications.

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