Overview

  • Product name

    Oil Red O (Lipid Stain) Solution
  • Product overview

    Oil Red O (Lipid Stain) Solution is intended for use in the histological visualization of fat cells and neutral fat. This kit may be used ONLY on frozen tissue sections, fresh smears, or touch preps. 


    We also sell solid Oil Red O stain ab146295 if you wish to prepare your own solution.

  • Notes

    Staining Interpretation

      

    Fat Cells   Red
    Neutral Fat     Red
    Nuclei Blue

     

    Control Tissue: Any frozen section containing fat.

  • Tested applications

    Suitable for: IHC-Frmore details

Properties

  • Storage instructions

    Store at room temperature. Please refer to protocols.
  • Components 100 tests
    Oil Red O Solution 1 x 125ml

Applications

Our Abpromise guarantee covers the use of ab223796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Images

  • Oil Red O (Lipid Stain) Solution (ab223796) staining lipid deposits (red) in frozen normal human skin. The dermis is unstained, whereas the hypodermis is stained, demonstrating dye specificity. Hematoxylin (blue) is used as a counterstain.  

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • During adipocyte differentiation, cells accumulate lipid. Oil Red O (Lipid Stain) Solution (ab223796) was used to visualize lipid in 3T3-L1 cells (left) and adipocyte-like cells (right). As expected, lipid accumulates in 3T3-L1 cells after differentiation into adipocyte-like cells.

Protocols

References

This product has been referenced in:

  • Jiao Y  et al. The Fto Gene Regulates the Proliferation and Differentiation of Pre-Adipocytes in Vitro. Nutrients 8:102 (2016). Read more (PubMed: 26907332) »
  • Patel AV  et al. Fatty acid synthase is a metabolic oncogene targetable in malignant peripheral nerve sheath tumors. Neuro Oncol 17:1599-608 (2015). Read more (PubMed: 26116612) »
See all 3 Publications for this product

Customer reviews and Q&As

I tried many different product to get staining for oil red O better and more clear but failed then I bought ab223796 - Oil Red O (Lipid Stain) Solution , as recommended in their datasheets and I never had any problem with it. I think its great product to use. I think this is best product to detect oil droplets during in vivo and in vitro studies

Abcam user community

Verified customer

Submitted Sep 05 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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