Recombinant
RabMAb

Anti-OIT3 antibody [EPR9349] (ab172973)

Overview

  • Product name
    Anti-OIT3 antibody [EPR9349]
    See all OIT3 primary antibodies
  • Description
    Rabbit monoclonal [EPR9349] to OIT3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human OIT3 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8WWZ8

  • Positive control
    • Human plasma, Human fetal liver and HepG2 lysates. Permeabilized HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9349
  • Isotype
    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab172973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Relevance
      OIT3 contains one EGF-like domain and one ZP domain. It may be involved in hepatocellular function and development. OIT3 is liver-specific and is expressed only in the hepatocytes; down-regulated in hepatocellular carcinoma (HCC) and HCC cell lines. There are two named isoforms.
    • Cellular localization
      Nucleus envelope. Can be secreted into blood.
    • Database links
    • Alternative names
      • Liver specific zona pellucida domain containing protein antibody
      • Liver specific ZP domain containing protein antibody
      • LZP antibody
      • Oncoprotein induced transcript 3 antibody

    Images

    • All lanes : Anti-OIT3 antibody [EPR9349] (ab172973) at 1/1000 dilution

      Lane 1 : Human Plasma
      Lane 2 : Human fetal liver lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • Flow cytometric analysis of permeabilized HepG2 cells labeling OIT3 with ab172973 at 1/100 diution  (red) or a rabbit IgG (negative) (green).

    References

    ab172973 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab172973.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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