Overview

  • Product name

  • Description

    Rabbit polyclonal to OLA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human OLA1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9NTK5

  • Positive control

    • IHC-P: Human colon carcinoma tissue. WB: HepG2 293T, A431, and HeLa whole cell lysates; Mouse brain lysate. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as GTPBP9

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-OLA1 antibody (ab229090) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 45 kDa

  • Anti-OLA1 antibody (ab229090) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded human colon carcinoma tissue stained for OLA1 using ab229090 at 1/250 dilution in immunohistochemical analysis.

  • Anti-OLA1 antibody (ab229090) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 45 kDa



    10% SDS-PAGE gel.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for OLA1 (green) using ab229090 at 1/200 dilution in ICC/IF. Cells were fixed in methanol.

    Blue: Hoechst 33342 staining.

References

ab229090 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229090.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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