Product nameOlanzapine, 5-HT, dopamine, histamine and muscarinic antagonist
DescriptionNon-selective 5-HT, dopamine, histamine and muscarinic antagonist
Biological description5-HT and D2 antagonist (Ki values are 4 (5-HT2A), 11 (5-HT2C) and 57 (5-HT3), 31 (D1), 11 (D2) and 27 nM (D4)). Additionally, displays activity at: M1, M2, M3, M4, H1, α1 and α2 receptors (Ki are 1.9 at (M1), 18 (M2), 25 (M3), 13 (M4), 19 (α1) and 7 nM (α2)). Atypical antipsychotic. Displays anxiolytic activity.
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Our Abpromise guarantee covers the use of ab120736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab32572 staining β catenin in SKNSH cells treated with olanzapine (ab120736), by ICC/IF. Increase in expression of β catenin correlates with increased concentration of olanzapine, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120736 (olanzapine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32572 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Sun T et al. Anxiolytic-like property of risperidone and olanzapine as examined in multiple measures of fear in rats. Pharmacol Biochem Behav 95:298-307 (2010). Read more (PubMed: 20167232) »
- Raedler TJ et al. In vivo olanzapine occupancy of muscarinic acetylcholine receptors in patients with schizophrenia. Neuropsychopharmacology 23:56-68 (2000). Read more (PubMed: 10869886) »
- Bymaster FP et al. Radioreceptor binding profile of the atypical antipsychotic olanzapine. Neuropsychopharmacology 14:87-96 (1996). Read more (PubMed: 8822531) »