Overview

  • Product name

    Anti-Olfactory Marker Protein/OMP antibody
    See all Olfactory Marker Protein/OMP primary antibodies
  • Description

    Rabbit polyclonal to Olfactory Marker Protein/OMP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Goat, Hamster
  • Immunogen

    Synthetic peptide corresponding to Rat Olfactory Marker Protein/OMP aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab102104)

  • Positive control

    • This antibody gave a positive signal in Mouse and Rat Olfactory Bulb Tissue Lysates.
  • General notes

     This product was previously labelled as Olfactory Marker Protein

     

Properties

Applications

Our Abpromise guarantee covers the use of ab87338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
IHC-FoFr 1/1000.

Target

  • Relevance

    Olfactory marker protein (OMP) is an abundant, 19kDa, cytosolic protein that is almost exclusively expressed in mature, functioning, olfactory neurons but not in the neural precursor basal cells. The OMP gene structure and protein sequence are highly conserved between mouse, rat and human. Its tissue specific expression in the receptor cells together with the results of the mouse knockout studies show that OMP represents a novel modulatory component of the odor detection/signal transduction cascade.
  • Cellular localization

    Cytoplasmic
  • Database links

  • Alternative names

    • Olfactory marker protein antibody
    • Olfactory neuronal specific protein antibody
    • OMP antibody

Images

  • IHC-FoFr image of Olfactory Marker Protein/OMP staining on mouse olfactor bulb using ab87338. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat.

    See Abreview

  • Anti-Olfactory Marker Protein/OMP antibody (ab87338) at 1 µg/ml + Rat Olfactory Bulb Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • Anti-Olfactory Marker Protein/OMP antibody (ab87338) at 1 µg/ml + Mouse Olfactory Bulb Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 18 kDa
    Observed band size: 18 kDa


    Exposure time: 16 minutes

References

This product has been referenced in:

  • Chandler JD  et al. Low-dose oral cadmium increases airway reactivity and lung neuronal gene expression in mice. Physiol Rep 4:N/A (2016). WB ; Mouse . Read more (PubMed: 27401458) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Olfactory neuronal precursor (OP6))
Permeabilization
Yes - 0.5% Triton-X in PBS
Specification
Olfactory neuronal precursor (OP6)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 37°C
Fixative
Paraformaldehyde

Ms. Joyce Noble

Verified customer

Submitted Feb 01 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Olfactory epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako citrate target retrieval buffer
Permeabilization
No
Specification
Olfactory epithelium
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 19 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Olfactory Epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako citrate antigen retrieval buffer
Permeabilization
No
Specification
Olfactory Epithelium
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5%
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 16 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain - Olfactory Bulb)
Specification
Brain - Olfactory Bulb
Fixative
Paraformaldehyde
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Mar 20 2012

Question
Answer

We use 5% BSA for blocking and diluting the primary antibody, which for some antibodies will give a stronger signal than 5% milk. So you may want to try that switch, if you are using milk to block and dilute. I also suggest simply using more antibody (e.g., 1/200) or loading more sample (e.g., 30 ug per lane), assuming the antibody has relatively weak affinity for the mouse protein compared to rat. I hope this helps. If you continue to have trouble, please contact us.

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