Anti-OLFM4 antibody (ab105861)
Key features and details
- Rabbit polyclonal to OLFM4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-OLFM4 antibody
See all OLFM4 primary antibodies -
Description
Rabbit polyclonal to OLFM4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Monkey -
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 400-450 (ASTGNMVISK LNDTTLQVLN TWYTKQYKPS ASNAFMVCGV LYATRTMNTR T) of Human OLFM4, conjugated to KLH.
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Positive control
- Human liver lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 57 kDa.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 57 kDa. |
Target
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Function
An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion. -
Tissue specificity
Expressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues. -
Sequence similarities
Contains 1 olfactomedin-like domain. -
Domain
The olfactomedin-like domain is involved in the interaction with cadherin. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 467367 Chimpanzee
- Entrez Gene: 515082 Cow
- Entrez Gene: 485470 Dog
- Entrez Gene: 10562 Human
- Entrez Gene: 380924 Mouse
- Entrez Gene: 290409 Rat
- Omim: 614061 Human
- SwissProt: Q6UX06 Human
see all -
Alternative names
- Antiapoptotic protein GW112 antibody
- bA209J19.1 antibody
- G-CSF-stimulated clone 1 protein antibody
see all
Images
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All lanes : Anti-OLFM4 antibody (ab105861) at 2 µg/ml
Lane 1 : Human liver lysate with no immunizing peptide
Lane 2 : Human liver lysate with immunizing peptide
Predicted band size: 57 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OLFM4 antibody (ab105861)ab105861, at 5 µg/ml, staining OLFM4 in formalin fixed, paraffin embedded colon tissue by Immunohistochemistry. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody followed by alkaline phosphatase-streptavidin and chromogen.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab105861 has not yet been referenced specifically in any publications.