Anti-OLFML2A antibody (ab75882)
Key features and details
- Rabbit polyclonal to OLFML2A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-OLFML2A antibody -
Description
Rabbit polyclonal to OLFML2A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 73 kDa.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 73 kDa. |
ICC/IF
1/500 - 1/1000. |
Target
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Relevance
OLFML2A contains one olfactomedin-like domain. Its exact function is unknown. There are three named isoforms produced by alternative splicing. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 169611 Human
- Entrez Gene: 241327 Mouse
- SwissProt: Q68BL7 Human
- SwissProt: Q8BHP7 Mouse
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Alternative names
- FLJ00237 antibody
- Olfactomedin like 2A antibody
- Olfactomedin like protein 2A antibody
see all
Images
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All lanes : Anti-OLFML2A antibody (ab75882) at 1/500 dilution
Lane 1 : extracts from RAW264.7 cells
Lane 2 : extracts from RAW264.7 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 73 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted? -
ab75882, at a dilution of 1/500-1/1000, staining OLFML2A in A549 cells by Immunofluorescence with (+) or without (-) immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab75882 has been referenced in 3 publications.
- Ma S et al. OLFML2A Downregulation Inhibits Glioma Proliferation Through Suppression of Wnt/β-Catenin Signaling. Front Oncol 11:717917 (2021). PubMed: 34650914
- Garza-Rodríguez ML et al. Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) profile expression in the retina of spotted gar (Lepisosteus oculatus) and bioinformatics mining. Fish Physiol Biochem 45:1575-1587 (2019). PubMed: 31111317
- Pérez-Ibave DC et al. Olfactomedin-like 2 A and B (OLFML2A and OLFML2B) expression profile in primates (human and baboon). Biol Res 49:44 (2016). ICC/IF ; Human . PubMed: 27821182