Overview

  • Product name

  • Description

    Rabbit polyclonal to Olig2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: CHIPseq, WB, IP, IHC-P, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant mouse Olig2

  • Positive control

    • Mouse brain whole cell lysate, PC12 whole cell lysate, brain (rat) tissue lysate. Glioblastoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab136253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
CHIPseq Use at an assay dependent concentration. PubMed: 26023283
WB 1/2500 - 1/5000. Predicted molecular weight: 32 kDa.
IP 1/500 - 1/1000.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.

Target

  • Function

    Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
  • Tissue specificity

    Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
  • Involvement in disease

    Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain

    The bHLH is essential for interaction with NKX2-2.
  • Cellular localization

    Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basic domain helix loop helix protein class B 1 antibody
    • Basic helix loop helix protein class B 1 antibody
    • BHLHB antibody
    • bHLHB1 antibody
    • bHLHe19 antibody
    • Class B basic helix loop helix protein 1 antibody
    • Class B basic helix-loop-helix protein 1 antibody
    • class E basic helix loop helix protein 19 antibody
    • Class E basic helix-loop-helix protein 19 antibody
    • Human protein kinase C binding protein RACK17 antibody
    • Olig2 antibody
    • OLIG2_HUMAN antibody
    • Oligo2 antibody
    • Oligodendrocyte lineage transcription factor 2 antibody
    • Oligodendrocyte specific bHLH transcription factor 2 antibody
    • Oligodendrocyte transcription factor 2 antibody
    • OTTHUMP00000067569 antibody
    • OTTHUMP00000067570 antibody
    • PRKCBP2 antibody
    • Protein kinase C binding protein 2 antibody
    • Protein kinase C binding protein RACK17 antibody
    • Protein kinase C-binding protein 2 antibody
    • Protein kinase C-binding protein RACK17 antibody
    • RACK17 antibody
    see all

Images

  • Immunohistochemical analysis of glioblastoma labelling Olig2 with ab136253 at 1/500 dilution. Tissue was pretreated with TE Buffer, pH 9.0.

References

This product has been referenced in:

  • Satoh J  et al. A Comprehensive Profile of ChIP-Seq-Based Olig2 Target Genes in Motor Neuron Progenitor Cells Suggests the Possible Involvement of Olig2 in the Pathogenesis of Amyotrophic Lateral Sclerosis. J Cent Nerv Syst Dis 7:1-14 (2015). CHIPseq . Read more (PubMed: 26023283) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Flow Cytometry
Sample
Mouse Cell (brain)
Permeabilization
Yes - Foxp3 staining buffer set (ThermoFischer, reference: 00-5523-00
Gating Strategy
CD45 - Olig2
Specification
brain
Preparation
Cell harvesting/tissue preparation method: Perfusion with PBS, enzymatic dissociation with accutase
Sample buffer: Foxp3 staining buffer set, ThermoFisher catalogue # 00-5523-00
Fixation
Paraformaldehyde

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Submitted Jan 30 2018

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