Anti-Olig2 antibody (ab136253)
Key features and details
- Rabbit polyclonal to Olig2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Olig2 antibody
See all Olig2 primary antibodies -
Description
Rabbit polyclonal to Olig2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant mouse Olig2
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.75% Glycine, 2.42% Tris -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab136253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. -
Tissue specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. -
Involvement in disease
Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
The bHLH is essential for interaction with NKX2-2. -
Cellular localization
Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. - Information by UniProt
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Database links
- Entrez Gene: 10215 Human
- Omim: 606386 Human
- SwissProt: Q13516 Human
- Unigene: 176977 Human
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Alternative names
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- BHLHB antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab136253 has been referenced in 16 publications.
- Iwasawa E et al. The Anti-Inflammatory Agent Bindarit Attenuates the Impairment of Neural Development through Suppression of Microglial Activation in a Neonatal Hydrocephalus Mouse Model. J Neurosci 42:1820-1844 (2022). PubMed: 34992132
- Zhou XH et al. Remimazolam induced cognitive dysfunction in mice via glutamate excitotoxicity. Transl Neurosci 13:104-115 (2022). PubMed: 35734308
- Qiao Y et al. Effects of Tibetan medicine metacinnabar (β-HgS) combined with imipramine or sertraline on depression-like symptoms in mice. Front Pharmacol 13:971243 (2022). PubMed: 36120298
- Sinha A et al. Phagocytic Activities of Reactive Microglia and Astrocytes Associated with Prion Diseases Are Dysregulated in Opposite Directions. Cells 10:N/A (2021). PubMed: 34359897
- Cirnaru MD et al. Unbiased identification of novel transcription factors in striatal compartmentation and striosome maturation. Elife 10:N/A (2021). PubMed: 34609283