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    olig2-antibody-ab136253.pdf

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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker
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Anti-Olig2 antibody (ab136253)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (11)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody (ab136253)

    Key features and details

    • Rabbit polyclonal to Olig2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Olig2 antibody [EPR2673] (ab109186)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Olig2 antibody
      See all Olig2 primary antibodies
    • Description

      Rabbit polyclonal to Olig2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant mouse Olig2

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituents: 0.75% Glycine, 2.42% Tris
    • Concentration information loading...
    • Purity

      Ammonium Sulphate Precipitation
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Cell Type Marker
      • Glia marker
      • Oligodendrocyte marker
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Stem Cells
      • Neural Stem Cells
      • Neuron Restricted Lineage
      • Neuroscience
      • Development

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • PC-12 nuclear extract lysate (ab14884)
      • Mouse brain tissue lysate - total protein (ab30151)
    • Recombinant Protein

      • Recombinant human Olig2 protein (ab169905)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab136253 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    Notes
    IHC-P
    1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Target

    • Function

      Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
    • Tissue specificity

      Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
    • Involvement in disease

      Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Domain

      The bHLH is essential for interaction with NKX2-2.
    • Cellular localization

      Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
    • Target information above from: UniProt accession Q13516 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10215 Human
      • Omim: 606386 Human
      • SwissProt: Q13516 Human
      • Unigene: 176977 Human
      • Alternative names

        • Basic domain helix loop helix protein class B 1 antibody
        • Basic helix loop helix protein class B 1 antibody
        • BHLHB antibody
        • bHLHB1 antibody
        • bHLHe19 antibody
        • Class B basic helix loop helix protein 1 antibody
        • Class B basic helix-loop-helix protein 1 antibody
        • class E basic helix loop helix protein 19 antibody
        • Class E basic helix-loop-helix protein 19 antibody
        • Human protein kinase C binding protein RACK17 antibody
        • Olig2 antibody
        • OLIG2_HUMAN antibody
        • Oligo2 antibody
        • Oligodendrocyte lineage transcription factor 2 antibody
        • Oligodendrocyte specific bHLH transcription factor 2 antibody
        • Oligodendrocyte transcription factor 2 antibody
        • OTTHUMP00000067569 antibody
        • OTTHUMP00000067570 antibody
        • PRKCBP2 antibody
        • Protein kinase C binding protein 2 antibody
        • Protein kinase C binding protein RACK17 antibody
        • Protein kinase C-binding protein 2 antibody
        • Protein kinase C-binding protein RACK17 antibody
        • RACK17 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody (ab136253)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody (ab136253)

        Immunohistochemical analysis of human glioblastoma labelling Olig2 with ab136253 at 1/500 dilution. Tissue was pretreated with TE Buffer, pH 9.0.

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (11)

      Publishing research using ab136253? Please let us know so that we can cite the reference in this datasheet.

      ab136253 has been referenced in 11 publications.

      • Kushwaha R  et al. Non-cell autonomous astrocyte-mediated neuronal toxicity in prion diseases. Acta Neuropathol Commun 9:22 (2021). PubMed: 33546775
      • Wegmann S  et al. Persistent repression of tau in the brain using engineered zinc finger protein transcription factors. Sci Adv 7:N/A (2021). PubMed: 33741591
      • Sternbach S  et al. The BHMT-betaine methylation pathway epigenetically modulates oligodendrocyte maturation. PLoS One 16:e0250486 (2021). PubMed: 33975330
      • Sinha A  et al. Phagocytic Activities of Reactive Microglia and Astrocytes Associated with Prion Diseases Are Dysregulated in Opposite Directions. Cells 10:N/A (2021). PubMed: 34359897
      • Benavente F  et al. Novel C1q receptor-mediated signaling controls neural stem cell behavior and neurorepair. Elife 9:N/A (2020). PubMed: 32894219
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review

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      Flow Cytometry abreview for Anti-Olig2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Flow Cytometry
      Sample
      Mouse Cell (brain)
      Permeabilization
      Yes - Foxp3 staining buffer set (ThermoFischer, reference: 00-5523-00
      Gating Strategy
      CD45 - Olig2
      Specification
      brain
      Preparation
      Cell harvesting/tissue preparation method: Perfusion with PBS, enzymatic dissociation with accutase
      Sample buffer: Foxp3 staining buffer set, ThermoFisher catalogue # 00-5523-00
      Fixation
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 30 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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