Product nameAnti-Olig2 antibody
See all Olig2 primary antibodies
DescriptionRabbit polyclonal to Olig2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Fr, IHC (PFA fixed)more details
Species reactivityReacts with: Human, Rhesus monkey
Predicted to work with: Mouse, Chicken
- Purchase matching WB positive control:Recombinant Human Olig2 protein
- WB: Jurkat cell lysate. IHC-Fr: Human brain tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab42453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).Can be blocked with Human Olig2 peptide (ab111666). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20025521|
|IHC-Fr||1/200. (see Abreview and notes)|
|IHC (PFA fixed)||1/1000.|
FunctionRequired for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
Tissue specificityExpressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
Involvement in diseaseNote=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
DomainThe bHLH is essential for interaction with NKX2-2.
Cellular localizationNucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
- Information by UniProt
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- BHLHB antibody
Anti-Olig2 antibody (ab42453) at 0.5 µg/ml (in 5% skim milk/PBS buffer) + Jurkat cell lysate
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 32 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
ab42453 at a 1/100 dilution staining Olig2 in human brain tissue (pontine base) by Immunohistochemistry (frozen sections) incubated for 1 hour at 20°C. Acetone fixed. Blocked with 10% milk for 30 minutes at 20°C. Secondary used at 1/200 pig polyclonal conjugated to biotin.
Note nuclear and cytoplasmic staining of oligodendrocytes in the white matter and neurons in the grey matter.
This product has been referenced in:
- Alamir H et al. In situ characterization of stem cells-like biomarkers in meningiomas. Cancer Cell Int 18:77 (2018). Read more (PubMed: 29849507) »
- Sharma A et al. Angiogenic Gene Signature Derived from Subtype Specific Cell Models Segregate Proneural and Mesenchymal Glioblastoma. Front Oncol 7:146 (2017). Read more (PubMed: 28744448) »