Anti-Olig2 antibody [EPR2673] (ab109186)
- Datasheet
- Specific References (16)
- Protocols
Overview
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Product nameAnti-Olig2 antibody [EPR2673]
See all Olig2 primary antibodies -
DescriptionRabbit monoclonal [EPR2673] to Olig2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Olig2 aa 250-350. The exact sequence is proprietary.
Database link: Q13516 -
Positive control
- Purchase matching WB positive control:Recombinant Human Olig2 protein
- Oligodendroglioma lysate, human glioma tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 1.50 x 10 -11 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR2673
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
Our Abpromise guarantee covers the use of ab109186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Predicted molecular weight: 32 kDa. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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FunctionRequired for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
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Tissue specificityExpressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
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Involvement in diseaseNote=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
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Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
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DomainThe bHLH is essential for interaction with NKX2-2.
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Cellular localizationNucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
- Information by UniProt
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Database links
- Entrez Gene: 10215 Human
- Entrez Gene: 50913 Mouse
- Entrez Gene: 304103 Rat
- Omim: 606386 Human
- SwissProt: Q13516 Human
- SwissProt: Q9EQW6 Mouse
- Unigene: 176977 Human
- Unigene: 37289 Mouse
see all -
Alternative names
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- BHLHB antibody
see all
Images
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![Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-7-ab109186WB3.jpg)
Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)All lanes : Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified)
All lanes : Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-6-ab109186WB2.jpg)
Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)Anti-Olig2 antibody [EPR2673] (ab109186) at 1/10000 dilution (purified) + Human oligodendroglioma lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-5-ab109186WB1.jpg)
Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-9-ab109186IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-8-ab109186IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Immunohistochemistry (Frozen sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-233582-ab109186ihcf.jpg)
Immunohistochemistry (Frozen sections) - Anti-Olig2 antibody [EPR2673] (ab109186)This image is courtesy of an Abreview submitted by Ruma Raha-ChowdhuryUnpurified ab109186 staining Olig2 in rat brain tissue sections by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in 0.1M PBS and blocked with 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/200 in PBS + 10% donkey serum) for 4 hours at 24°C. An Alexa Fluor® 488-conjugated anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei stained with Hoechst (blue).
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![Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/Olig2-Primary-antibodies-ab109186-1.jpg)
Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)Anti-Olig2 antibody [EPR2673] (ab109186) at 1/1000 dilution (unpurified) + Oligodendroglioma lysate at 10 µg
Performed under reducing conditions.
Predicted band size: 32 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/Olig2-Primary-antibodies-ab109186-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)Immunohistochemical staining of Olig2 in human glioma tissue with ab109186 at a dilution of 1/100.
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![Other - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/Olig2-Primary-antibodies-ab109186-3.jpg)
Other - Anti-Olig2 antibody [EPR2673] (ab109186)
References
This product has been referenced in:
- Foditsch EE et al. A new technique for minimal invasive complete spinal cord injury in minipigs. Acta Neurochir (Wien) 160:459-465 (2018). Read more (PubMed: 29330575) »
- Henrich MT et al. A53T-a-synuclein overexpression in murine locus coeruleus induces Parkinson's disease-like pathology in neurons and glia. Acta Neuropathol Commun 6:39 (2018). Read more (PubMed: 29747690) »
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