Recombinant Anti-Olig2 antibody [EPR2673] (ab109186)
- Datasheet
- References (31)
- Protocols
Overview
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Product name
Anti-Olig2 antibody [EPR2673]
See all Olig2 primary antibodies -
Description
Rabbit monoclonal [EPR2673] to Olig2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Olig2 aa 250-350. The exact sequence is proprietary.
Database link: Q13516 -
Positive control
- Purchase matching WB positive control:Recombinant Human Olig2 protein
- WB: Oligodendroglioma lysate, human fetal brain lysate, mouse brain lysate IHC-Fr: Rat brain tissue. IHC-P: Rat and human cerebral cortex tissue; Human glioma tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.50 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2673 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab109186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000. Predicted molecular weight: 32 kDa. | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Function
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. -
Tissue specificity
Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. -
Involvement in disease
Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Domain
The bHLH is essential for interaction with NKX2-2. -
Cellular localization
Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. - Information by UniProt
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Database links
- Entrez Gene: 10215 Human
- Entrez Gene: 50913 Mouse
- Entrez Gene: 304103 Rat
- Omim: 606386 Human
- SwissProt: Q13516 Human
- SwissProt: Q9EQW6 Mouse
- Unigene: 176977 Human
- Unigene: 37289 Mouse
see all -
Alternative names
- Basic domain helix loop helix protein class B 1 antibody
- Basic helix loop helix protein class B 1 antibody
- BHLHB antibody
see all
Images
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![Immunohistochemistry (Frozen sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-323731-anti-olig2-antibody-epr2673-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Olig2 antibody [EPR2673] (ab109186)Connolly N.P. et al PLoS One. 2017 Mar 30;12(3):e0174557. doi: 10.1371/journal.pone.0174557. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunofluorescent labeling of brain tumors reveals brain invasion and reactive processes at the tumor margin
Oligodendroglial tumor cells (Olig2+) invading through and within the reactive astrocytosis (GFAP+) in the tumor margin.
Cryosections of rat brain were stained for Olig2 using ab109186 at 1/100 dilution.
(From Figure 6 of Connolly et al)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-355127-anti-olig2-antibody-immunohistochemistry-mouse-brain-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)This image was courtesy of an annoymous AbreviewFormaldehyde-fixed mouse brain tissue stained for Olig2 using ab109186 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was a Horse Radish Peroxidase conjugated Dako Envision Rabbit antibody.
Antigen retrieval: Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
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![Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-243957-anti-olig2-antibody-epr2673-western-blot.jpg)
Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)All lanes : Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified)
All lanes : Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Immunohistochemistry (Frozen sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-233582-anti-olig2-antibody-epr2673-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Olig2 antibody [EPR2673] (ab109186)This image is courtesy of an Abreview submitted by Ruma Raha-ChowdhuryUnpurified ab109186 staining Olig2 in rat brain tissue sections by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in 0.1M PBS and blocked with 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/200 in PBS + 10% donkey serum) for 4 hours at 24°C. An Alexa Fluor® 488-conjugated anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei stained with Hoechst (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-244115-anti-olig2-antibody-epr2673-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Other - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-121782-anti-olig2-antibody-epr2673.jpg)
Other - Anti-Olig2 antibody [EPR2673] (ab109186) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-355128-anti-olig2-antibody-immunohistochemistry-cynomolgus-brain-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)This image was courtesy of an annonymous AbreviewFormaldehyde-fixed Cynomolgus monkey brain tissue stained for Olig2 using ab109186 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was a Horse Radish Peroxidase conjugated Dako Envision Rabbit antibody.
Antigen Retrieval: Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
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![Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-243956-anti-olig2-antibody-epr2673-western-blot.jpg)
Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)Anti-Olig2 antibody [EPR2673] (ab109186) at 1/10000 dilution (purified) + Human oligodendroglioma lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-243955-anti-olig2-antibody-epr2673-western-blot.jpg)
Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-244114-anti-olig2-antibody-epr2673-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset. -
![Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-121784-anti-olig2-antibody-epr2673-western-blot.jpg)
Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)Anti-Olig2 antibody [EPR2673] (ab109186) at 1/1000 dilution (unpurified) + Oligodendroglioma lysate at 10 µg
Performed under reducing conditions.
Predicted band size: 32 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)](https://www.abcam.com/ps/products/109/ab109186/Images/ab109186-121783-anti-olig2-antibody-epr2673-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)Immunohistochemical staining of Olig2 in human glioma tissue with ab109186 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
References
This product has been referenced in:
- Wong YL et al. eIF2B activator prevents neurological defects caused by a chronic integrated stress response. Elife 8:N/A (2019). Read more (PubMed: 30624206) »
- Lim Y et al. Arx Expression Suppresses Ventralization of the Developing Dorsal Forebrain. Sci Rep 9:226 (2019). Read more (PubMed: 30659230) »
