Overview

  • Product name

    Anti-Olig2 antibody [EPR2673]
    See all Olig2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2673] to Olig2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Olig2 aa 250-350. The exact sequence is proprietary.
    Database link: Q13516

  • Positive control

  • General notes

      

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 32 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
    • Tissue specificity

      Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
    • Involvement in disease

      Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
    • Sequence similarities

      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Domain

      The bHLH is essential for interaction with NKX2-2.
    • Cellular localization

      Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
    • Information by UniProt
    • Database links

    • Alternative names

      • Basic domain helix loop helix protein class B 1 antibody
      • Basic helix loop helix protein class B 1 antibody
      • BHLHB antibody
      • bHLHB1 antibody
      • bHLHe19 antibody
      • Class B basic helix loop helix protein 1 antibody
      • Class B basic helix-loop-helix protein 1 antibody
      • class E basic helix loop helix protein 19 antibody
      • Class E basic helix-loop-helix protein 19 antibody
      • Human protein kinase C binding protein RACK17 antibody
      • Olig2 antibody
      • OLIG2_HUMAN antibody
      • Oligo2 antibody
      • Oligodendrocyte lineage transcription factor 2 antibody
      • Oligodendrocyte specific bHLH transcription factor 2 antibody
      • Oligodendrocyte transcription factor 2 antibody
      • OTTHUMP00000067569 antibody
      • OTTHUMP00000067570 antibody
      • PRKCBP2 antibody
      • Protein kinase C binding protein 2 antibody
      • Protein kinase C binding protein RACK17 antibody
      • Protein kinase C-binding protein 2 antibody
      • Protein kinase C-binding protein RACK17 antibody
      • RACK17 antibody
      see all

    Images

    • Immunofluorescent labeling of brain tumors reveals brain invasion and reactive processes at the tumor margin

      Oligodendroglial tumor cells (Olig2+) invading through and within the reactive astrocytosis (GFAP+) in the tumor margin.

      Cryosections of rat brain were stained for Olig2 using ab109186 at 1/100 dilution.

      (From Figure 6 of Connolly et al)

    • Formaldehyde-fixed mouse brain tissue stained for Olig2 using ab109186 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was a Horse Radish Peroxidase conjugated Dako Envision Rabbit antibody.

      Antigen retrieval: Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

    • All lanes : Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified)

      All lanes : Mouse brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Unpurified ab109186 staining Olig2 in rat brain tissue sections by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in 0.1M PBS and blocked with 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody (1/200 in PBS + 10% donkey serum) for 4 hours at 24°C. An Alexa Fluor® 488-conjugated anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei stained with Hoechst (blue).

      See Abreview

    • Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Formaldehyde-fixed Cynomolgus monkey brain tissue stained for Olig2 using ab109186 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was a Horse Radish Peroxidase conjugated Dako Envision Rabbit antibody.

      Antigen Retrieval: Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

    • Anti-Olig2 antibody [EPR2673] (ab109186) at 1/10000 dilution (purified) + Human oligodendroglioma lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Anti-Olig2 antibody [EPR2673] (ab109186) at 1/1000 dilution (unpurified) + Oligodendroglioma lysate at 10 µg

      Performed under reducing conditions.

      Predicted band size: 32 kDa

    • Immunohistochemical staining of Olig2 in human glioma tissue with ab109186 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    See all 31 Publications for this product

    Customer reviews and Q&As

    1-10 of 10 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Rat Tissue sections (Brain Sections)
    Specification
    Brain Sections
    Permeabilization
    Yes - 0.1M PBS with 3% TritonX100
    Fixative
    Paraformaldehyde

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Oct 02 2014

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain section)
    Specification
    Brain section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 14 2014

    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain 40 µm slice)
    Specification
    brain 40 µm slice

    Abcam user community

    Verified customer

    Submitted Mar 20 2019

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Oligodendrocytes in brain frozen sections)
    Permeabilization
    Yes - 0.2% triton in PBS
    Specification
    Oligodendrocytes in brain frozen sections
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Diego Perez Rodriguez

    Verified customer

    Submitted Dec 03 2018

    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain

    Abcam user community

    Verified customer

    Submitted Oct 17 2018

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse neural stem cell)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    40 µg
    Specification
    mouse neural stem cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jun 12 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus monkey Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 10 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 09 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH6
    Permeabilization
    No
    Specification
    Spinal cord
    Blocking step
    Ultratek HRP (anti-polyvalent) kit, Scytek laboratories as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Formaldehyde

    Sara Belluco

    Verified customer

    Submitted Apr 07 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1
    Permeabilization
    No
    Specification
    Brain
    Fixative
    formalin

    Mr. Emmanuel Bouchaert

    Verified customer

    Submitted Jan 03 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up