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  1. Link

    olig2-antibody-epr2673-ab109186.pdf

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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker
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RecombinantRabMAb

Recombinant Anti-Olig2 antibody [EPR2673] (ab109186)

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Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
  • OI-RD Scanning - Anti-Olig2 antibody [EPR2673] (ab109186)
  • Anti-Olig2 antibody [EPR2673] (ab109186)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2673] to Olig2
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-Olig2 antibody [EPR2673]
    See all Olig2 primary antibodies
  • Description

    Rabbit monoclonal [EPR2673] to Olig2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Purchase matching WB positive control:Recombinant Human Olig2 protein
    • WB: Oligodendroglioma lysate, human fetal brain lysate, mouse and rat brain lysate IHC-P: Rat and human cerebral cortex tissue; Human glioma tissue. ICC/IF: Rat primary glia cells. Primary mouse neurons/glia, DIV14 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.50 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2673
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-Olig2 antibody [EPR2673] - BSA and Azide free (ab220796)
    • Alexa Fluor® 488 Anti-Olig2 antibody [EPR2673] (ab225099)
    • Alexa Fluor® 647 Anti-Olig2 antibody [EPR2673] (ab225100)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Olig2 protein (ab169905)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
Use a concentration of 1 - 5 µg/ml.
WB (3)
1/2000. Predicted molecular weight: 32 kDa.
IHC-P (6)
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
Use a concentration of 1 - 5 µg/ml.
WB
1/2000. Predicted molecular weight: 32 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
  • Tissue specificity

    Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.
  • Involvement in disease

    Note=A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Domain

    The bHLH is essential for interaction with NKX2-2.
  • Cellular localization

    Nucleus. Cytoplasm. The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.
  • Target information above from: UniProt accession Q13516 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10215 Human
    • Entrez Gene: 50913 Mouse
    • Entrez Gene: 304103 Rat
    • Omim: 606386 Human
    • SwissProt: Q13516 Human
    • SwissProt: Q9EQW6 Mouse
    • Unigene: 176977 Human
    • Unigene: 37289 Mouse
    • Alternative names

      • Basic domain helix loop helix protein class B 1 antibody
      • Basic helix loop helix protein class B 1 antibody
      • BHLHB antibody
      • bHLHB1 antibody
      • bHLHe19 antibody
      • Class B basic helix loop helix protein 1 antibody
      • Class B basic helix-loop-helix protein 1 antibody
      • class E basic helix loop helix protein 19 antibody
      • Class E basic helix-loop-helix protein 19 antibody
      • Human protein kinase C binding protein RACK17 antibody
      • Olig2 antibody
      • OLIG2_HUMAN antibody
      • Oligo2 antibody
      • Oligodendrocyte lineage transcription factor 2 antibody
      • Oligodendrocyte specific bHLH transcription factor 2 antibody
      • Oligodendrocyte transcription factor 2 antibody
      • OTTHUMP00000067569 antibody
      • OTTHUMP00000067570 antibody
      • PRKCBP2 antibody
      • Protein kinase C binding protein 2 antibody
      • Protein kinase C binding protein RACK17 antibody
      • Protein kinase C-binding protein 2 antibody
      • Protein kinase C-binding protein RACK17 antibody
      • RACK17 antibody
      see all

    Images

    • Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
      Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
      Different batches of ab109186 were tested on Mouse brain lysate at 0.1 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 32 kDa.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling NeuN with ab177487 at 1/100 dilution (B), SOX1 with ab242125 at 1/100 dilution (C) and Olig2 with ab109186 at 1/100 dilution (D). Anti-Rabbit and Mouse Polymer HRP was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Heat mediated antigen retrieval (Leica ER2, PH9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibodies from the previous round, to avoid any cross-reactivity. 

      Panel A: merged staining of anti- NeuN (green, Opal™520), anti-SOX1 (red, Opal™570) and anti- Olig2 (yellow, Opal™690).

      Panel B: anti-NeuN stands for neurons.

      Panel C: anti-SOX1 stained on neural progenitors.

      Panel D: anti-Olig2 stained on oligodendrocyte.

      The section was incubated in three rounds of staining: in the order of ab177487, ab242125 and ab109186 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope

    • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] (ab109186)

      ab109186 staining Olig2 in primary mouse neurons/glia, DIV14 (prepared from E18 mouse hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab109186 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] (ab109186)

      ab109186 staining Olig2 in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab109186 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 100% methanol (5 min).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunocytochemistry/ Immunofluorescence - Anti-Olig2 antibody [EPR2673] (ab109186)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary glia cell cells labelling Olig2 with ab109186 at 1/100 (1.23 µg/mL) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing nuclear staining in rat primary glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)This image was courtesy of an annoymous Abreview

      Formaldehyde-fixed mouse brain tissue stained for Olig2 using ab109186 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was a Horse Radish Peroxidase conjugated Dako Envision Rabbit antibody.

      Antigen retrieval: Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

      See Abreview

    • Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
      Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
      All lanes : Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified)

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
      Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
      Anti-Olig2 antibody [EPR2673] (ab109186) at 1/10000 dilution (purified) + Human oligodendroglioma lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
      Western blot - Anti-Olig2 antibody [EPR2673] (ab109186)
      Anti-Olig2 antibody [EPR2673] (ab109186) at 1/2000 dilution (purified) + Human fetal brain tissue lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 32 kDa
      Observed band size: 32 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab109186 at a working dilution of 1/100. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] (ab109186)

      Immunohistochemical staining of Olig2 in human glioma tissue with ab109186 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-Olig2 antibody [EPR2673] (ab109186)
      OI-RD Scanning - Anti-Olig2 antibody [EPR2673] (ab109186)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Olig2 antibody [EPR2673] (ab109186)
      Anti-Olig2 antibody [EPR2673] (ab109186)

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (94)

    Publishing research using ab109186? Please let us know so that we can cite the reference in this datasheet.

    ab109186 has been referenced in 94 publications.

    • Chen Z  et al. Cell surface GRP78 regulates BACE2 via lysosome-dependent manner to maintain mesenchymal phenotype of glioma stem cells. J Exp Clin Cancer Res 40:20 (2021). PubMed: 33413577
    • Wang Z  et al. Hypoxia-induced PLOD1 overexpression contributes to the malignant phenotype of glioblastoma via NF-?B signaling. Oncogene N/A:N/A (2021). PubMed: 33420370
    • Bo-Yin Z  et al. Unlocking the Recovery Potential: JMJD3 Inhibition-Mediated SAPK/JNK Signaling Inactivation Supports Endogenous Oligodendrocyte-Lineage Commitment Post Mammalian Spinal Cord Injury. Neurochem Res 46:792-803 (2021). PubMed: 33428096
    • Costa B  et al. A Set of Cell Lines Derived from a Genetic Murine Glioblastoma Model Recapitulates Molecular and Morphological Characteristics of Human Tumors. Cancers (Basel) 13:N/A (2021). PubMed: 33435218
    • Wang Q  et al. Combined transcriptomic and lipidomic analysis reveals aberrant lipid metabolism in central nervous system hemangioblastomas. Sci Rep 11:1314 (2021). PubMed: 33446752
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 22 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Rat Tissue sections (Brain Sections)
    Specification
    Brain Sections
    Permeabilization
    Yes - 0.1M PBS with 3% TritonX100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    Submitted Oct 02 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain section)
    Specification
    Brain section
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 14 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Cat Tissue sections (Optic nerve)
    Permeabilization
    Yes - 0.2% TritonX-100
    Specification
    Optic nerve
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 18 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution (S1699) - 20min @ ~90oC
    Permeabilization
    Yes - Wash buffer, blocking solution and secondary diluent was 1X TBS with 0.1% Tween-20
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    10% neutral buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 24 2022

    Western blot abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Non-reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 11 2022

    Flow Cytometry abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Brain purified nuclei)
    Permeabilization
    Yes - PBS 1x, 5% BSA, 0.5% Tween-20
    Gating Strategy
    NeuN negative nuclei were gated on and posteriorly Olig2 population
    Specification
    Brain purified nuclei
    Fixation
    Non-fixed
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 10 2022

    Immunohistochemistry free floating abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (Cerebellum)
    Specification
    Cerebellum
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    Submitted Apr 26 2021

    Immunohistochemistry free floating abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 26 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brain tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC1
    Permeabilization
    No
    Specification
    Brain tumor
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Masahiro Hirata

    Verified customer

    Submitted Feb 22 2021

    Western blot abreview for Anti-Olig2 antibody [EPR2673]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Cerebellum)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    50 µg
    Specification
    Cerebellum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 06 2021

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