Application
Flow Cytometry
Sample
Mouse Cell (Brain purified nuclei)
Permeabilization
Yes - PBS 1x, 5% BSA, 0.5% Tween-20
Gating Strategy
NeuN negative nuclei were gated on and posteriorly Olig2 population
Specification
Brain purified nuclei
Fixation
Non-fixed
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS 1x, 5% BSA, 0.5% Tween-20
Dilution
1/200
Secondary antibody
Dilution
1/1500
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondar
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
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Submitted Jan 10 2022