Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution (S1699) - 20min @ ~90oC
Permeabilization
Yes - Wash buffer, blocking solution and secondary diluent was 1X TBS with 0.1% Tween-20
Specification
Brain
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
10% neutral buffered formalin
Other product details
Incubation time
30 minute(s) · Temperature: 22°C · Diluent: Dako Antibody Diluent (S3022)
Concentration
0.25 µg/ml
Secondary antibody
Concentration
5 µg/ml
Name
Non-Abcam antibody was used: AffiniPure Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Submitted Jan 24 2022